1h1z
From Proteopedia
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| - | [[Image:1h1z.jpg|left|200px]] | + | [[Image:1h1z.jpg|left|200px]] |
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| - | '''THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE AND ZINC''' | + | {{Structure |
| + | |PDB= 1h1z |SIZE=350|CAPTION= <scene name='initialview01'>1h1z</scene>, resolution 3.40Å | ||
| + | |SITE= <scene name='pdbsite=ZNA:So4+Binding+Site+For+Chain+B'>ZNA</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribulose-phosphate_3-epimerase Ribulose-phosphate 3-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.1 5.1.3.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE AND ZINC''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H1Z is a [ | + | 1H1Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H1Z OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice., Jelakovic S, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 2003 Feb 7;326(1):127-35. PMID:[http:// | + | Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice., Jelakovic S, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 2003 Feb 7;326(1):127-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12547196 12547196] |
[[Category: Oryza sativa]] | [[Category: Oryza sativa]] | ||
[[Category: Ribulose-phosphate 3-epimerase]] | [[Category: Ribulose-phosphate 3-epimerase]] | ||
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[[Category: oxidative pentose phosphate pathway]] | [[Category: oxidative pentose phosphate pathway]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:31:08 2008'' |
Revision as of 09:31, 20 March 2008
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| , resolution 3.40Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Ribulose-phosphate 3-epimerase, with EC number 5.1.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE AND ZINC
Overview
Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized after EDTA treatment and structurally elucidated by X-ray diffraction to 1.9A resolution. A prominent Zn(2+) site at the active center was established in a soaking experiment. The structure was compared with that of the EDTA-treated crystalline enzyme from the chloroplasts of potato plant leaves showing some structural differences, in particular the "closed" state of a strongly conserved mobile loop covering the substrate at its putative binding site. The previous proposal for the active center was confirmed and the most likely substrate binding position and conformation was derived from the locations of the bound zinc and sulfate ions and of three water molecules. Assuming that the bound zinc ion is an integral part of the enzyme, a reaction mechanism involving a well-stabilized cis-enediolate intermediate is suggested.
About this Structure
1H1Z is a Single protein structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.
Reference
Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice., Jelakovic S, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 2003 Feb 7;326(1):127-35. PMID:12547196
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