1h34
From Proteopedia
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| - | [[Image:1h34.jpg|left|200px]] | + | [[Image:1h34.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR''' | + | {{Structure |
| + | |PDB= 1h34 |SIZE=350|CAPTION= <scene name='initialview01'>1h34</scene>, resolution 2.04Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H34 is a [ | + | 1H34 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA]. |
==Reference== | ==Reference== | ||
| - | In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:[http:// | + | In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554963 12554963] |
[[Category: Phaseolus lunatus]] | [[Category: Phaseolus lunatus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine protease inhibitor]] | [[Category: serine protease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:31:31 2008'' |
Revision as of 09:31, 20 March 2008
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| , resolution 2.04Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR
Overview
SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
About this Structure
1H34 is a Single protein structure of sequence from Phaseolus lunatus. Full crystallographic information is available from OCA.
Reference
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:12554963
Page seeded by OCA on Thu Mar 20 11:31:31 2008
