1gxw
From Proteopedia
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| - | [[ | + | ==the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate== |
| + | <StructureSection load='1gxw' size='340' side='right' caption='[[1gxw]], [[Resolution|resolution]] 2.18Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1gxw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GXW FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fj3|1fj3]], [[1fjo|1fjo]], [[1fjq|1fjq]], [[1fjt|1fjt]], [[1fju|1fju]], [[1fjv|1fjv]], [[1fjw|1fjw]], [[1hyt|1hyt]], [[1lna|1lna]], [[1lnb|1lnb]], [[1lnc|1lnc]], [[1lnd|1lnd]], [[1lne|1lne]], [[1lnf|1lnf]], [[1qf0|1qf0]], [[1qf1|1qf1]], [[1qf2|1qf2]], [[1thl|1thl]], [[1tli|1tli]], [[1tlp|1tlp]], [[1tlx|1tlx]], [[1tmn|1tmn]], [[1trl|1trl]], [[2tli|2tli]], [[2tlx|2tlx]], [[2tmn|2tmn]], [[3tli|3tli]], [[3tmn|3tmn]], [[4tli|4tli]], [[4tln|4tln]], [[4tmn|4tmn]], [[5tli|5tli]], [[5tln|5tln]], [[5tmn|5tmn]], [[6tli|6tli]], [[6tmn|6tmn]], [[7tli|7tli]], [[7tln|7tln]], [[8tli|8tli]], [[8tln|8tln]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gxw RCSB], [http://www.ebi.ac.uk/pdbsum/1gxw PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxw_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A new crystallization protocol for thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus is presented. After dissolving the protein in the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were obtained following the salting-in method. Crystal cell parameters are isomorphous with those previously reported from DMSO/CsCl mixtures. The new SCN(-) crystal structure has been analyzed. It shows the presence of one thiocyanate ion in the catalytic site and several rearrangements in the S(1) and S(2) subsites. These results are in agreement with the measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly poor in low ionic strength solutions, increases dramatically in the presence of several neutral salts. The results reported here suggest possible explanations for the solubility increase and for the inhibitory effects of high SCN(-) concentrations on thermolysin activity. | ||
| - | + | The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate.,Gaucher JF, Selkti M, Prange T, Tomas A Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500<ref>PMID:12454500</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Thermolysin|Thermolysin]] | *[[Thermolysin|Thermolysin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bacillus thermoproteolyticus]] | [[Category: Bacillus thermoproteolyticus]] | ||
[[Category: Thermolysin]] | [[Category: Thermolysin]] | ||
Revision as of 16:51, 29 September 2014
the 2.2 A resolution structure of thermolysin crystallized in presence of potassium thiocyanate
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