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1s1i

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{{Large structure}}
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==Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.==
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{{STRUCTURE_1s1i| PDB=1s1i | SCENE= }}
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<StructureSection load='1s1i' size='340' side='right' caption='[[1s1i]], [[Resolution|resolution]] 11.70&Aring;' scene=''>
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===Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.===
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== Structural highlights ==
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{{ABSTRACT_PUBMED_14976550}}
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<table><tr><td colspan='2'>[[1s1i]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S1I FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1k5x|1k5x]], [[1k5y|1k5y]], [[1mms|1mms]], [[1mzp|1mzp]], [[1ffk|1ffk]], [[1fjf|1fjf]], [[1fka|1fka]], [[1gix|1gix]], [[1giy|1giy]], [[1nou|1nou]], [[1s1h|1s1h]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s1i RCSB], [http://www.ebi.ac.uk/pdbsum/1s1i PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s1/1s1i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
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==About this Structure==
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Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.,Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jorgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J EMBO J. 2004 Mar 10;23(5):1008-19. Epub 2004 Feb 19. PMID:14976550<ref>PMID:14976550</ref>
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[[1s1i]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1I OCA].
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==See Also==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Ribosomal protein L1|Ribosomal protein L1]]
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</div>
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*[[Ribosomal protein L10|Ribosomal protein L10]]
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*[[Ribosomal protein L11|Ribosomal protein L11]]
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*[[Ribosomal protein L13|Ribosomal protein L13]]
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*[[Ribosomal protein L15|Ribosomal protein L15]]
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*[[Ribosomal protein L16|Ribosomal protein L16]]
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*[[Ribosomal protein L17|Ribosomal protein L17]]
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*[[Ribosomal protein L18|Ribosomal protein L18]]
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*[[Ribosomal protein L19|Ribosomal protein L19]]
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*[[Ribosomal protein L2|Ribosomal protein L2]]
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*[[Ribosomal protein L23|Ribosomal protein L23]]
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*[[Ribosomal protein L25|Ribosomal protein L25]]
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*[[Ribosomal protein L28|Ribosomal protein L28]]
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*[[Ribosomal protein L29|Ribosomal protein L29]]
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*[[Ribosomal protein L3|Ribosomal protein L3]]
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*[[Ribosomal protein L31|Ribosomal protein L31]]
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*[[Ribosomal protein L32|Ribosomal protein L32]]
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*[[Ribosomal protein L34|Ribosomal protein L34]]
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*[[Ribosomal protein L35|Ribosomal protein L35]]
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*[[Ribosomal protein L4|Ribosomal protein L4]]
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*[[Ribosomal protein L41|Ribosomal protein L41]]
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*[[Ribosomal protein L42|Ribosomal protein L42]]
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*[[Ribosomal protein L43|Ribosomal protein L43]]
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*[[Ribosomal protein L44|Ribosomal protein L44]]
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*[[Ribosomal protein L5|Ribosomal protein L5]]
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*[[Ribosomal protein L6|Ribosomal protein L6]]
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*[[Ribosomal protein L8|Ribosomal protein L8]]
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*[[Ribosomal protein L9|Ribosomal protein L9]]
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==Reference==
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==See Also==
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<ref group="xtra">PMID:014976550</ref><references group="xtra"/>
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Andersen, G R.]]
[[Category: Andersen, G R.]]

Revision as of 17:04, 29 September 2014

Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.

1s1i, resolution 11.70Å

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