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1h4i

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[[Image:1h4i.gif|left|200px]]<br /><applet load="1h4i" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1h4i.gif|left|200px]]
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caption="1h4i, resolution 1.94&Aring;" />
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'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''<br />
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{{Structure
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|PDB= 1h4i |SIZE=350|CAPTION= <scene name='initialview01'>1h4i</scene>, resolution 1.94&Aring;
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|SITE= <scene name='pdbsite=AC1:Pqq+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq+Binding+Site+For+Chain+C'>AC3</scene> and <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+C'>AC4</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=PQQ:PYRROLOQUINOLINE QUINONE'>PQQ</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8]
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|GENE=
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}}
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'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1H4I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PQQ:'>PQQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Known structural/functional Sites: <scene name='pdbsite=AC1:Pqq+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq+Binding+Site+For+Chain+C'>AC3</scene> and <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+C'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA].
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1H4I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA].
==Reference==
==Reference==
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The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7735834 7735834]
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The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7735834 7735834]
[[Category: Alcohol dehydrogenase (acceptor)]]
[[Category: Alcohol dehydrogenase (acceptor)]]
[[Category: Methylobacterium extorquens]]
[[Category: Methylobacterium extorquens]]
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[[Category: quinoprotein]]
[[Category: quinoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:32:04 2008''

Revision as of 09:32, 20 March 2008

Image:1h4i.gif


PDB ID 1h4i

Drag the structure with the mouse to rotate
, resolution 1.94Å
Sites: , , and
Ligands: and
Activity: Alcohol dehydrogenase (acceptor), with EC number 1.1.99.8
Coordinates: save as pdb, mmCIF, xml



METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE


Overview

BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic group, requires Ca2+ for activity and uses cytochrome cL as its electron acceptor. Low-resolution structures of MDH have already been determined. RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from Methylobacterium extorquens has now been determined at 1.94 A with an R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an eight-fold radial symmetry, with its eight beta-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulphide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group.

About this Structure

1H4I is a Protein complex structure of sequences from Methylobacterium extorquens. Full crystallographic information is available from OCA.

Reference

The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:7735834

Page seeded by OCA on Thu Mar 20 11:32:04 2008

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