1h5w
From Proteopedia
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| - | [[Image:1h5w.jpg|left|200px]] | + | [[Image:1h5w.jpg|left|200px]] |
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| - | '''2.1A BACTERIOPHAGE PHI-29 CONNECTOR''' | + | {{Structure |
| + | |PDB= 1h5w |SIZE=350|CAPTION= <scene name='initialview01'>1h5w</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''2.1A BACTERIOPHAGE PHI-29 CONNECTOR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H5W is a [ | + | 1H5W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5W OCA]. |
==Reference== | ==Reference== | ||
| - | Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage phi29 connector particle., Guasch A, Pous J, Ibarra B, Gomis-Ruth FX, Valpuesta JM, Sousa N, Carrascosa JL, Coll M, J Mol Biol. 2002 Jan 25;315(4):663-76. PMID:[http:// | + | Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage phi29 connector particle., Guasch A, Pous J, Ibarra B, Gomis-Ruth FX, Valpuesta JM, Sousa N, Carrascosa JL, Coll M, J Mol Biol. 2002 Jan 25;315(4):663-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11812138 11812138] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio phage f237]] | [[Category: Vibrio phage f237]] | ||
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[[Category: virus]] | [[Category: virus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:32:41 2008'' |
Revision as of 09:32, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
2.1A BACTERIOPHAGE PHI-29 CONNECTOR
Overview
The three-dimensional crystal structure of the bacteriophage phi29 connector has been solved and refined to 2.1A resolution. This 422 kDa oligomeric protein connects the head of the phage to its tail and translocates the DNA into the prohead during packaging. Each monomer has an elongated shape and is composed of a central, mainly alpha-helical domain that includes a three-helix bundle, a distal alpha/beta domain and a proximal six-stranded SH3-like domain. The protomers assemble into a 12-mer, propeller-like, super-structure with a 35 A wide central channel. The surface of the channel is mainly electronegative, but it includes two lysine rings 20 A apart. On the external surface of the particle a hydrophobic belt extends to the concave area below the SH3-like domain, which forms a crown that retains the particle in the head. The lipophilic belt contacts the non-matching symmetry vertex of the capsid and forms a bearing for the connector rotation. The structure suggests a translocation mechanism in which the longitudinal displacement of the DNA along its axis is coupled to connector spinning.
About this Structure
1H5W is a Single protein structure of sequence from Vibrio phage f237. Full crystallographic information is available from OCA.
Reference
Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage phi29 connector particle., Guasch A, Pous J, Ibarra B, Gomis-Ruth FX, Valpuesta JM, Sousa N, Carrascosa JL, Coll M, J Mol Biol. 2002 Jan 25;315(4):663-76. PMID:11812138
Page seeded by OCA on Thu Mar 20 11:32:41 2008
Categories: Single protein | Vibrio phage f237 | Carrascosa, J L. | Coll, M. | Gomis-Ruth, F X. | Guasch, A. | Ibarra, B. | Pous, J. | Sousa, N. | Valpuesta, J M. | MPD | Connector | Helix bundle | Portal | Sh3-like | Virus
