1w9m

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[[Image:1w9m.png|left|200px]]
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==AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL==
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<StructureSection load='1w9m' size='340' side='right' caption='[[1w9m]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1w9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W9M FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FS2:FE-S-O+HYBRID+CLUSTER'>FS2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1e1d|1e1d]], [[1e2u|1e2u]], [[1e9v|1e9v]], [[1gnt|1gnt]], [[1oa1|1oa1]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w9m RCSB], [http://www.ebi.ac.uk/pdbsum/1w9m PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/1w9m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-wavelength anomalous diffraction data collected in the vicinity of the Fe K absorption edge. Although the overall protein structure is essentially the same as that previously obtained, it shows that the nature of the hybrid cluster has particular differences when isolated and crystallized in the absence of oxygen and this provides insight into the structural features associated with changes in the oxidation state. A comparison between HCPs and carbon monoxide dehydrogenases (CoDs) shows that they possess a similar fold and that the dehydrogenases have a related cluster at the equivalent HCP hybrid cluster position. This helps to understand the nature of the hybrid cluster and to predict a dimeric structure for class 3 HCPs, which lack the N-terminal region.
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{{STRUCTURE_1w9m| PDB=1w9m | SCENE= }}
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Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution.,Aragao D, Mitchell EP, Frazao CF, Carrondo MA, Lindley PF Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):665-74. Epub 2008, May 14. PMID:18560155<ref>PMID:18560155</ref>
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===AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18560155}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1w9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9M OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018560155</ref><ref group="xtra">PMID:012764602</ref><references group="xtra"/>
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[[Category: Desulfovibrio vulgaris]]
[[Category: Desulfovibrio vulgaris]]
[[Category: Aragao, D.]]
[[Category: Aragao, D.]]

Revision as of 18:57, 29 September 2014

AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL

1w9m, resolution 1.35Å

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