1h9d
From Proteopedia
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| - | [[Image:1h9d.gif|left|200px]] | + | [[Image:1h9d.gif|left|200px]] |
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| - | '''AML1/CBF-BETA/DNA COMPLEX''' | + | {{Structure |
| + | |PDB= 1h9d |SIZE=350|CAPTION= <scene name='initialview01'>1h9d</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= AML1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CBFB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''AML1/CBF-BETA/DNA COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H9D is a [ | + | 1H9D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9D OCA]. |
==Reference== | ==Reference== | ||
| - | The leukemia-associated AML1 (Runx1)--CBF beta complex functions as a DNA-induced molecular clamp., Bravo J, Li Z, Speck NA, Warren AJ, Nat Struct Biol. 2001 Apr;8(4):371-8. PMID:[http:// | + | The leukemia-associated AML1 (Runx1)--CBF beta complex functions as a DNA-induced molecular clamp., Bravo J, Li Z, Speck NA, Warren AJ, Nat Struct Biol. 2001 Apr;8(4):371-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11276260 11276260] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:34:01 2008'' |
Revision as of 09:34, 20 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | AML1 (Homo sapiens), CBFB (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
AML1/CBF-BETA/DNA COMPLEX
Contents |
Overview
We have determined the structure, at 2.6 A resolution, of the AML1 (Runx1) Runt domain--CBF beta--DNA ternary complex, the most common target for mutations in human leukemia. The structure reveals that the Runt domain DNA binding mechanism is unique within the p53 family of transcription factors. The extended C-terminal 'tail' and 'wing' elements adopt a specific DNA-bound conformation that clamps the phosphate backbone between the major and minor grooves of the distorted B-form DNA recognition site. Furthermore, the extended 'tail' mediates most of the NF-kappa B/Rel-like base-specific contacts in the major groove. The structure clearly explains the molecular basis for the loss of DNA binding function of the Runt domain--CBF beta complex as a consequence of the human disease-associated mutations in leukemogenesis and cleidocranial dysplasia.
Disease
Known diseases associated with this structure: Leukemia, acute myeloid OMIM:[151385], Myeloid leukemia, acute, M4Eo subtype OMIM:[121360], Platelet disorder, familial, with associated myeloid malignancy OMIM:[151385], Rheumatoid arthritis, susceptibility to OMIM:[151385]
About this Structure
1H9D is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The leukemia-associated AML1 (Runx1)--CBF beta complex functions as a DNA-induced molecular clamp., Bravo J, Li Z, Speck NA, Warren AJ, Nat Struct Biol. 2001 Apr;8(4):371-8. PMID:11276260
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