1h9o
From Proteopedia
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| - | [[Image:1h9o.gif|left|200px]] | + | [[Image:1h9o.gif|left|200px]] |
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| - | '''PHOSPHATIDYLINOSITOL 3-KINASE, P85-ALPHA SUBUNIT: C-TERMINAL SH2 DOMAIN COMPLEXED WITH A TYR751 PHOSPHOPEPTIDE FROM THE PDGF RECEPTOR, CRYSTAL STRUCTURE AT 1.79 A''' | + | {{Structure |
| + | |PDB= 1h9o |SIZE=350|CAPTION= <scene name='initialview01'>1h9o</scene>, resolution 1.79Å | ||
| + | |SITE= <scene name='pdbsite=PTR:Ptr+Binding+Site'>PTR</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''PHOSPHATIDYLINOSITOL 3-KINASE, P85-ALPHA SUBUNIT: C-TERMINAL SH2 DOMAIN COMPLEXED WITH A TYR751 PHOSPHOPEPTIDE FROM THE PDGF RECEPTOR, CRYSTAL STRUCTURE AT 1.79 A''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H9O is a [ | + | 1H9O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9O OCA]. |
==Reference== | ==Reference== | ||
| - | NMR trial models: experiences with the colicin immunity protein Im7 and the p85alpha C-terminal SH2-peptide complex., Pauptit RA, Dennis CA, Derbyshire DJ, Breeze AL, Weston SA, Rowsell S, Murshudov GN, Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1397-404. Epub, 2001 Sep 21. PMID:[http:// | + | NMR trial models: experiences with the colicin immunity protein Im7 and the p85alpha C-terminal SH2-peptide complex., Pauptit RA, Dennis CA, Derbyshire DJ, Breeze AL, Weston SA, Rowsell S, Murshudov GN, Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1397-404. Epub, 2001 Sep 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11567151 11567151] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:34:10 2008'' |
Revision as of 09:34, 20 March 2008
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| , resolution 1.79Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHOSPHATIDYLINOSITOL 3-KINASE, P85-ALPHA SUBUNIT: C-TERMINAL SH2 DOMAIN COMPLEXED WITH A TYR751 PHOSPHOPEPTIDE FROM THE PDGF RECEPTOR, CRYSTAL STRUCTURE AT 1.79 A
Contents |
Overview
Two cases of successful molecular replacement using NMR trial models are presented. One is the crystal structure of the Escherichia coli colicin immunity protein Im7; the other is a heretofore unreported crystal structure of a specific PDGF receptor-derived peptide complex of the carboxy-terminal SH2 domain from the p85alpha subunit of human phosphatidylinositol 3-OH kinase. In both cases, molecular replacement was non-trivial. Success was achieved using trial models that consisted of an ensemble of NMR structures from which the more flexible portions had been excised. Use of maximum-likelihood refinement proved critical to be able to refine the poor starting models. The challenges typical of the use of NMR trial models in molecular replacement are discussed.
Disease
Known diseases associated with this structure: Myelomonocytic leukemia, chronic OMIM:[173410], Myeloproliferative disorder with eosinophilia OMIM:[173410]
About this Structure
1H9O is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
NMR trial models: experiences with the colicin immunity protein Im7 and the p85alpha C-terminal SH2-peptide complex., Pauptit RA, Dennis CA, Derbyshire DJ, Breeze AL, Weston SA, Rowsell S, Murshudov GN, Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1397-404. Epub, 2001 Sep 21. PMID:11567151
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