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1han

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[[Image:1han.gif|left|200px]]<br /><applet load="1han" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1han.gif|left|200px]]
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caption="1han, resolution 1.9&Aring;" />
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'''CRYSTAL STRUCTURE OF THE BIPHENYL-CLEAVING EXTRADIOL DIOXYGENASE FROM A PCB-DEGRADING PSEUDOMONAD'''<br />
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{{Structure
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|PDB= 1han |SIZE=350|CAPTION= <scene name='initialview01'>1han</scene>, resolution 1.9&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> and <scene name='pdbligand=TBU:TERTIARY-BUTYL ALCOHOL'>TBU</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Biphenyl-2,3-diol_1,2-dioxygenase Biphenyl-2,3-diol 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.39 1.13.11.39]
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|GENE= BPHC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292 Burkholderia cepacia])
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}}
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'''CRYSTAL STRUCTURE OF THE BIPHENYL-CLEAVING EXTRADIOL DIOXYGENASE FROM A PCB-DEGRADING PSEUDOMONAD'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1HAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia] with <scene name='pdbligand=FE:'>FE</scene> and <scene name='pdbligand=TBU:'>TBU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Biphenyl-2,3-diol_1,2-dioxygenase Biphenyl-2,3-diol 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.39 1.13.11.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAN OCA].
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1HAN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAN OCA].
==Reference==
==Reference==
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Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad., Han S, Eltis LD, Timmis KN, Muchmore SW, Bolin JT, Science. 1995 Nov 10;270(5238):976-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7481800 7481800]
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Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad., Han S, Eltis LD, Timmis KN, Muchmore SW, Bolin JT, Science. 1995 Nov 10;270(5238):976-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7481800 7481800]
[[Category: Biphenyl-2,3-diol 1,2-dioxygenase]]
[[Category: Biphenyl-2,3-diol 1,2-dioxygenase]]
[[Category: Burkholderia cepacia]]
[[Category: Burkholderia cepacia]]
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[[Category: extradiol dioxygenase]]
[[Category: extradiol dioxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:34:32 2008''

Revision as of 09:34, 20 March 2008

Image:1han.gif


PDB ID 1han

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: and
Gene: BPHC (Burkholderia cepacia)
Activity: Biphenyl-2,3-diol 1,2-dioxygenase, with EC number 1.13.11.39
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE BIPHENYL-CLEAVING EXTRADIOL DIOXYGENASE FROM A PCB-DEGRADING PSEUDOMONAD


Overview

Polychlorinated biphenyls (PCBs) typify a class of stable aromatic pollutants that are targeted by bioremediation strategies. In the aerobic degradation of biphenyl by bacteria, the key step of ring cleavage is catalyzed by an Fe(II)-dependent extradiol dioxygenase. The crystal structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB-degrading strain of Pseudomonas cepacia has been determined at 1.9 angstrom resolution. The monomer comprises amino- and carboxyl-terminal domains. Structural homology between and within the domains reveals evolutionary relationships within the extradiol dioxygenase family. The iron atom has five ligands in square pyramidal geometry: one glutamate and two histidine side chains, and two water molecules.

About this Structure

1HAN is a Single protein structure of sequence from Burkholderia cepacia. Full crystallographic information is available from OCA.

Reference

Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad., Han S, Eltis LD, Timmis KN, Muchmore SW, Bolin JT, Science. 1995 Nov 10;270(5238):976-80. PMID:7481800

Page seeded by OCA on Thu Mar 20 11:34:32 2008

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