1uut

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[[Image:1uut.png|left|200px]]
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==THE NUCLEASE DOMAIN OF ADENO-ASSOCIATED VIRUS REP COMPLEXED WITH THE RBE' STEMLOOP OF THE VIRAL INVERTED TERMINAL REPEAT==
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<StructureSection load='1uut' size='340' side='right' caption='[[1uut]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uut]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UUT FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m55|1m55]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uut OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uut RCSB], [http://www.ebi.ac.uk/pdbsum/1uut PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uut_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.
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{{STRUCTURE_1uut| PDB=1uut | SCENE= }}
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The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces.,Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F Mol Cell. 2004 Feb 13;13(3):403-14. PMID:14967147<ref>PMID:14967147</ref>
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===THE NUCLEASE DOMAIN OF ADENO-ASSOCIATED VIRUS REP COMPLEXED WITH THE RBE' STEMLOOP OF THE VIRAL INVERTED TERMINAL REPEAT===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_14967147}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1uut]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUT OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:014967147</ref><references group="xtra"/>
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[[Category: Adeno-associated virus - 5]]
[[Category: Adeno-associated virus - 5]]
[[Category: Dyda, F.]]
[[Category: Dyda, F.]]

Revision as of 19:48, 29 September 2014

THE NUCLEASE DOMAIN OF ADENO-ASSOCIATED VIRUS REP COMPLEXED WITH THE RBE' STEMLOOP OF THE VIRAL INVERTED TERMINAL REPEAT

1uut, resolution 2.00Å

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OCA

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