1tkw

Revision as of 20:27, 29 September 2014

The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR

Structural highlights

1tkw is a 2 chain structure with sequence from Brassica rapa subsp. rapa and Populus nigra. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1hcz, 5pcy, 2pcf
Gene:	PETE (Populus nigra), PETA (Brassica rapa subsp. rapa)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The orientation of poplar plastocyanin in the complex with turnip cytochrome f has been determined by rigid-body calculations using restraints from paramagnetic NMR measurements. The results show that poplar plastocyanin interacts with cytochrome f with the hydrophobic patch of plastocyanin close to the heme region on cytochrome f and via electrostatic interactions between the charged patches on both proteins. Plastocyanin is tilted relative to the orientation reported for spinach plastocyanin, resulting in a longer distance between iron and copper (13.9 A). With increasing ionic strength, from 0.01 to 0.11 M, all observed chemical-shift changes decrease uniformly, supporting the idea that electrostatic forces contribute to complex formation. There is no indication for a rearrangement of the transient complex in this ionic strength range, contrary to what had been proposed earlier on the basis of kinetic data. By decreasing the pH from pH 7.7 to pH 5.5, the complex is destabilized. This may be attributed to the protonation of the conserved acidic patches or the copper ligand His87 in poplar plastocyanin, which are shown to have similar pK(a) values. The results are interpreted in a two-step model for complex formation.

The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH.,Lange C, Cornvik T, Diaz-Moreno I, Ubbink M Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. Epub 2004 Dec 29. PMID:15863096^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lange C, Cornvik T, Diaz-Moreno I, Ubbink M. The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH. Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. Epub 2004 Dec 29. PMID:15863096 doi:10.1016/j.bbabio.2004.12.002

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tkw"

@@ Line 1: / Line 1: @@
-{{STRUCTURE_1tkw|  PDB=1tkw  |  SCENE=  }}
+==The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR==
-===The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR===
+<StructureSection load='1tkw' size='340' side='right' caption='[[1tkw]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
-{{ABSTRACT_PUBMED_15863096}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1tkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brassica_rapa_subsp._rapa Brassica rapa subsp. rapa] and [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TKW FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hcz|1hcz]], [[5pcy|5pcy]], [[2pcf|2pcf]]</td></tr>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PETE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3691 Populus nigra]), PETA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=51350 Brassica rapa subsp. rapa])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tkw RCSB], [http://www.ebi.ac.uk/pdbsum/1tkw PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tkw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The orientation of poplar plastocyanin in the complex with turnip cytochrome f has been determined by rigid-body calculations using restraints from paramagnetic NMR measurements. The results show that poplar plastocyanin interacts with cytochrome f with the hydrophobic patch of plastocyanin close to the heme region on cytochrome f and via electrostatic interactions between the charged patches on both proteins. Plastocyanin is tilted relative to the orientation reported for spinach plastocyanin, resulting in a longer distance between iron and copper (13.9 A). With increasing ionic strength, from 0.01 to 0.11 M, all observed chemical-shift changes decrease uniformly, supporting the idea that electrostatic forces contribute to complex formation. There is no indication for a rearrangement of the transient complex in this ionic strength range, contrary to what had been proposed earlier on the basis of kinetic data. By decreasing the pH from pH 7.7 to pH 5.5, the complex is destabilized. This may be attributed to the protonation of the conserved acidic patches or the copper ligand His87 in poplar plastocyanin, which are shown to have similar pK(a) values. The results are interpreted in a two-step model for complex formation.
-==About this Structure==
+The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH.,Lange C, Cornvik T, Diaz-Moreno I, Ubbink M Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. Epub 2004 Dec 29. PMID:15863096<ref>PMID:15863096</ref>
-[[1tkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brassica_rapa_subsp._rapa Brassica rapa subsp. rapa] and [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Cytochrome f|Cytochrome f]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:015863096</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Brassica rapa subsp. rapa]]
 [[Category: Populus nigra]]