1ud1

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[[Image:1ud1.png|left|200px]]
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==Crystal structure of proglycinin mutant C88S==
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<StructureSection load='1ud1' size='340' side='right' caption='[[1ud1]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ud1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UD1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UD1 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fxz|1fxz]], [[1ucx|1ucx]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ud1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ud1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ud1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ud1 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ud/1ud1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The constituent subunits of seed storage protein 11S globulin have two disulfide bonds that are common among 11S globulins from legume and nonlegume seeds. In the case of the A1aB1b subunit of soybean 11S globulin, glycinin, Cys12-Cys45 and Cys88-Cys298 are observed by X-ray crystallography. The significance of these two disulfide bonds for structural stability was investigated by mutagenesis of Cys12 to Gly and of Cys88 to Ser. The disulfide bond-deficient mutants C12G and C88S could form the correct conformations identical to that of the wild-type proglycinin except in the vicinities of the mutation sites C12 and C88 as shown by their crystal structures. Thermal stability monitored by differential scanning calorimetry of the mutants indicated that the contribution of these disulfide bonds to the thermal stability of proglycinin A1aB1b is low, although there is a small difference in the extent of the contribution between the two disulfide bonds (Cys12-Cys45 &gt; Cys88-Cys298). The contribution of Cys88-Cys298 to the resistance of proglycinin A1aB1b to proteinase digestion is higher than that of Cys12-Cys45. Possible effects of structure on the different properties of C12G and C88S are discussed.
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{{STRUCTURE_1ud1| PDB=1ud1 | SCENE= }}
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Crystal structures and structural stabilities of the disulfide bond-deficient soybean proglycinin mutants C12G and C88S.,Adachi M, Okuda E, Kaneda Y, Hashimoto A, Shutov AD, Becker C, Muntz K, Utsumi S J Agric Food Chem. 2003 Jul 30;51(16):4633-9. PMID:14705889<ref>PMID:14705889</ref>
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===Crystal structure of proglycinin mutant C88S===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_14705889}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1ud1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UD1 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:014705889</ref><references group="xtra"/>
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[[Category: Glycine max]]
[[Category: Glycine max]]
[[Category: Adachi, M.]]
[[Category: Adachi, M.]]

Revision as of 21:40, 29 September 2014

Crystal structure of proglycinin mutant C88S

1ud1, resolution 3.10Å

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