1hml
From Proteopedia
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- | [[Image:1hml.gif|left|200px]] | + | [[Image:1hml.gif|left|200px]] |
- | + | ||
- | '''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE''' | + | {{Structure |
+ | |PDB= 1hml |SIZE=350|CAPTION= <scene name='initialview01'>1hml</scene>, resolution 1.7Å | ||
+ | |SITE= | ||
+ | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
+ | |ACTIVITY= [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] | ||
+ | |GENE= | ||
+ | }} | ||
+ | |||
+ | '''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE''' | ||
+ | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
- | 1HML is a [ | + | 1HML is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HML OCA]. |
==Reference== | ==Reference== | ||
- | Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:[http:// | + | Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8366079 8366079] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Lactose synthase]] | [[Category: Lactose synthase]] | ||
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[[Category: calcium-binding protein]] | [[Category: calcium-binding protein]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:39:05 2008'' |
Revision as of 09:39, 20 March 2008
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, resolution 1.7Å | |||||||
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Ligands: | , and | ||||||
Activity: | Lactose synthase, with EC number 2.4.1.22 | ||||||
Coordinates: | save as pdb, mmCIF, xml |
ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE
Overview
It has been proposed that the binding of Zn2+ to alpha-lactalbumin switches the conformation to one akin to a state intermediate in the folding of the protein. However, the high resolution x-ray crystal structure of human alpha-lactalbumin-Zn2+ complex at 1.7-A resolution (pH 7.6) does not reveal any significant change in conformation from the native state. The Zn2+ ion binds specifically in the "cleft" of alpha-lactalbumin (the region which forms the active site of the homologous protein lysozyme). This may suggest a possible role for Zn2+ binding in lactose synthase complex. The coordination of the Zn2+ ion involves a symmetry-related molecule in the crystal, the crystal contacts being stabilized by a SO4(2-) ion bound at the interface between three molecules.
About this Structure
1HML is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:8366079
Page seeded by OCA on Thu Mar 20 11:39:05 2008
Categories: Homo sapiens | Lactose synthase | Single protein | Acharya, K R. | Ren, J. | Stuart, D I. | CA | SO4 | ZN | Calcium-binding protein