1hml

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1hml.gif|left|200px]]<br /><applet load="1hml" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1hml.gif|left|200px]]
-
caption="1hml, resolution 1.7&Aring;" />
+
 
-
'''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE'''<br />
+
{{Structure
 +
|PDB= 1hml |SIZE=350|CAPTION= <scene name='initialview01'>1hml</scene>, resolution 1.7&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22]
 +
|GENE=
 +
}}
 +
 
 +
'''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1HML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HML OCA].
+
1HML is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HML OCA].
==Reference==
==Reference==
-
Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8366079 8366079]
+
Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8366079 8366079]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Lactose synthase]]
[[Category: Lactose synthase]]
Line 22: Line 31:
[[Category: calcium-binding protein]]
[[Category: calcium-binding protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:48 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:39:05 2008''

Revision as of 09:39, 20 March 2008


PDB ID 1hml

Drag the structure with the mouse to rotate
, resolution 1.7Å
Ligands: , and
Activity: Lactose synthase, with EC number 2.4.1.22
Coordinates: save as pdb, mmCIF, xml



ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE


Overview

It has been proposed that the binding of Zn2+ to alpha-lactalbumin switches the conformation to one akin to a state intermediate in the folding of the protein. However, the high resolution x-ray crystal structure of human alpha-lactalbumin-Zn2+ complex at 1.7-A resolution (pH 7.6) does not reveal any significant change in conformation from the native state. The Zn2+ ion binds specifically in the "cleft" of alpha-lactalbumin (the region which forms the active site of the homologous protein lysozyme). This may suggest a possible role for Zn2+ binding in lactose synthase complex. The coordination of the Zn2+ ion involves a symmetry-related molecule in the crystal, the crystal contacts being stabilized by a SO4(2-) ion bound at the interface between three molecules.

About this Structure

1HML is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:8366079

Page seeded by OCA on Thu Mar 20 11:39:05 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools