2jba

From Proteopedia

Revision as of 15:24, 30 October 2007

PHOB RESPONSE REGULATOR RECEIVER DOMAIN CONSTITUTIVELY-ACTIVE DOUBLE MUTANT D53A AND Y102C.

Overview

The PhoR/PhoB two-component system is a key regulatory protein network, enabling Escherichia coli to respond to inorganic phosphate (Pi), starvation conditions by turning on Pho regulon genes for more efficient, Pi uptake and use of alternative phosphorus sources. Under environmental, Pi depletion, the response regulator (RR) component, PhoB, is, phosphorylated at the receiver domain (RD), a process that requires Mg(2+), bound at the active site. Phosphorylation of the RD relieves the, inhibition of the PhoB effector domain (ED), a DNA-binding region that, binds to Pho regulon promoters to activate transcription. The molecular, details of the activation are proposed to involve dimerization of the RD, and a conformational change in the RD detected by the ED. The structure of, the PhoB RD ... [(full description)]

About this Structure

2JBA is a [Single protein] structure of sequence from [Escherichia coli] with NA and TRS as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

The X-ray crystal structures of two constitutively active mutants of the Escherichia coli PhoB receiver domain give insights into activation., Arribas-Bosacoma R, Kim SK, Ferrer-Orta C, Blanco AG, Pereira PJ, Gomis-Ruth FX, Wanner BL, Coll M, Sola M, J Mol Biol. 2007 Feb 16;366(2):626-41. Epub 2006 Nov 14. PMID:17182055

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