2f5t

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[[Image:2f5t.png|left|200px]]
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==Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB==
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<StructureSection load='2f5t' size='340' side='right' caption='[[2f5t]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2f5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F5T FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f5t RCSB], [http://www.ebi.ac.uk/pdbsum/2f5t PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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TrmB is an alpha-glucoside-sensing transcriptional regulator controlling two operons encoding maltose/trehalose and maltodextrin ABC transporters of Pyrococcus furiosus. The crystal structure of an N-terminal truncated derivative of TrmB (amino acids 2-109 deleted; TrmB(delta2-109)) was solved at 1.5 A resolution. This protein has lost its DNA binding domain but has retained its sugar recognition site. The structure represents a novel sugar-binding fold. TrmB(delta2-109) bound maltose, glucose, sucrose, and maltotriose, exhibiting Kd values of 6.8, 25, 34, and 160 microM, respectively. TrmB(delta2-109) behaved as a monomer in dilute buffer solution in contrast to the full-length protein, which is a dimer. Co-crystallization with bound maltose identified a binding site involving seven amino acid residues: Ser229, Asn305, Gly320, Met321, Val324, Ile325, and Glu326. Six of these residues interact with the nonreducing glucosyl residue of maltose. The nonreducing glucosyl residue is shared by all substrates bound to TrmB, suggesting it as a common recognition motif.
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{{STRUCTURE_2f5t| PDB=2f5t | SCENE= }}
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Crystal structure of the sugar binding domain of the archaeal transcriptional regulator TrmB.,Krug M, Lee SJ, Diederichs K, Boos W, Welte W J Biol Chem. 2006 Apr 21;281(16):10976-82. Epub 2006 Feb 10. PMID:16473881<ref>PMID:16473881</ref>
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===Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16473881}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2f5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5T OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:016473881</ref><references group="xtra"/>
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[[Category: Thermococcus litoralis]]
[[Category: Thermococcus litoralis]]
[[Category: Boos, W.]]
[[Category: Boos, W.]]

Revision as of 02:28, 30 September 2014

Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB

2f5t, resolution 1.45Å

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