1hwq
From Proteopedia
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| - | [[Image:1hwq.gif|left|200px]] | + | [[Image:1hwq.gif|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP''' | + | {{Structure |
| + | |PDB= 1hwq |SIZE=350|CAPTION= <scene name='initialview01'>1hwq</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HWQ is a [ | + | 1HWQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA]. |
==Reference== | ==Reference== | ||
| - | A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http:// | + | A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350168 11350168] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dieckmann, T.]] | [[Category: Dieckmann, T.]] | ||
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[[Category: vs ribozyme]] | [[Category: vs ribozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:42:29 2008'' |
Revision as of 09:42, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP
Overview
The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
About this Structure
1HWQ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168
Page seeded by OCA on Thu Mar 20 11:42:29 2008
