1hzn
From Proteopedia
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| - | [[Image:1hzn.gif|left|200px]] | + | [[Image:1hzn.gif|left|200px]] |
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| - | '''NMR SOLUTION STRUCTURE OF THE THIRD EXTRACELLULAR LOOP OF THE CHOLECYSTOKININ A RECEPTOR''' | + | {{Structure |
| + | |PDB= 1hzn |SIZE=350|CAPTION= <scene name='initialview01'>1hzn</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> and <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR SOLUTION STRUCTURE OF THE THIRD EXTRACELLULAR LOOP OF THE CHOLECYSTOKININ A RECEPTOR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HZN is a [ | + | 1HZN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZN OCA]. |
==Reference== | ==Reference== | ||
| - | Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin A receptor., Giragossian C, Mierke DF, Biochemistry. 2001 Apr 3;40(13):3804-9. PMID:[http:// | + | Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin A receptor., Giragossian C, Mierke DF, Biochemistry. 2001 Apr 3;40(13):3804-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11300760 11300760] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Giragossian, C.]] | [[Category: Giragossian, C.]] | ||
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[[Category: hormone/growth factor]] | [[Category: hormone/growth factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:43:35 2008'' |
Revision as of 09:43, 20 March 2008
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR SOLUTION STRUCTURE OF THE THIRD EXTRACELLULAR LOOP OF THE CHOLECYSTOKININ A RECEPTOR
Overview
The interaction of the C-terminal octapeptide of cholecystokinin, CCK-8, with the third extracellular loop of human cholecystokinin-A receptor, CCK(A)-R(329-357), has been probed by high-resolution NMR and extensive computer simulations. The structure of CCK(A)-R(329-357) in the presence of dodecylphosphocholine micelles consists of three alpha-helices, with the first and third corresponding to the extracellular ends of transmembrane (TM) helices 6 and 7. The central helix, residues W335-R345, is found to lie on the zwitterionic surface. Titration with CCK-8 produces a stable complex with a number of intermolecular NOEs between the C-terminus of the ligand (Trp(30), Met(31), Asp(32)) and the interface of TM6 and the third extracellular loop (N333, A334, Y338) of the receptor fragment. The mode of ligand binding based on these intermolecular NOEs is in agreement with a number of published findings from receptor mutagenesis and photoaffinity cross-linking. Utilizing these ligand/receptor points of interaction, the structural features of CCK(A)-R(329-357), and also the structures of CCK-8 and CCK(A)-R(1-47) previously determined, extensive molecular dynamics simulations of the CCK-8/CCK(A)-R complex were carried out. The results provide unique insight into the molecular interactions and forces important for the binding of CCK-8 to CCK(A)-R.
About this Structure
1HZN is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin A receptor., Giragossian C, Mierke DF, Biochemistry. 2001 Apr 3;40(13):3804-9. PMID:11300760
Page seeded by OCA on Thu Mar 20 11:43:35 2008
