2j42

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2j42" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2j42.png|left|200px]]
+
==LOW QUALITY CRYSTAL STRUCTURE OF THE TRANSPORT COMPONENT C2-II OF THE C2-TOXIN FROM CLOSTRIDIUM BOTULINUM==
 +
<StructureSection load='2j42' size='340' side='right' caption='[[2j42]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2j42]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J42 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J42 FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j42 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j42 RCSB], [http://www.ebi.ac.uk/pdbsum/2j42 PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j4/2j42_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
C2 toxin from Clostridium botulinum is composed of the enzyme component C2-I, which ADP-ribosylates actin, and the binding and translocation component C2-II, responsible for the interaction with eukaryotic cell receptors and the following endocytosis. Three C2-I crystal structures at resolutions of up to 1.75 A are presented together with a crystal structure of C2-II at an appreciably lower resolution and a model of the prepore formed by fragment C2-IIa. The C2-I structure was determined at pH 3.0 and at pH 6.1. The structural differences are small, indicating that C2-I does not unfold, even at a pH value as low as 3.0. The ADP-ribosyl transferase activity of C2-I was determined for alpha and beta/gamma-actin and related to that of Iota toxin and of mutant S361R of C2-I that introduced the arginine observed in Iota toxin. The substantial activity differences between alpha and beta/gamma-actin cannot be explained by the protein structures currently available. The structure of the transport component C2-II at pH 4.3 was established by molecular replacement using a model of the protective antigen of anthrax toxin at pH 6.0. The C-terminal receptor-binding domain of C2-II could not be located but was present in the crystals. It may be mobile. The relative orientation and positions of the four other domains of C2-II do not differ much from those of the protective antigen, indicating that no large conformational changes occur between pH 4.3 and pH 6.0. A model of the C2-IIa prepore structure was constructed based on the corresponding assembly of the protective antigen. It revealed a surprisingly large number of asparagine residues lining the pore. The interaction between C2-I and C2-IIa and the translocation of C2-I into the target cell are discussed.
-
{{STRUCTURE_2j42| PDB=2j42 | SCENE= }}
+
Structure and action of the binary C2 toxin from Clostridium botulinum.,Schleberger C, Hochmann H, Barth H, Aktories K, Schulz GE J Mol Biol. 2006 Dec 8;364(4):705-15. Epub 2006 Sep 5. PMID:17027031<ref>PMID:17027031</ref>
-
===LOW QUALITY CRYSTAL STRUCTURE OF THE TRANSPORT COMPONENT C2-II OF THE C2-TOXIN FROM CLOSTRIDIUM BOTULINUM===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_17027031}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2j42]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J42 OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:017027031</ref><references group="xtra"/>
+
[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
[[Category: Aktories, K.]]
[[Category: Aktories, K.]]

Revision as of 08:09, 30 September 2014

LOW QUALITY CRYSTAL STRUCTURE OF THE TRANSPORT COMPONENT C2-II OF THE C2-TOXIN FROM CLOSTRIDIUM BOTULINUM

2j42, resolution 3.13Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j42"
Views
Personal tools
Navigation
Toolbox