2ft0

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[[Image:2ft0.png|left|200px]]
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==Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA==
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<StructureSection load='2ft0' size='340' side='right' caption='[[2ft0]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_cft073 Escherichia coli cft073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FT0 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fs5|2fs5]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">wecd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=199310 Escherichia coli CFT073])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ft0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ft0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ft0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ft0 PDBsum], [http://www.topsan.org/Proteins/BSGI/2ft0 TOPSAN]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/2ft0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enterobacterial common antigen (ECA) is a polysaccharide found on the outer membrane of virtually all gram-negative enteric bacteria and consists of three sugars, N-acetyl-d-glucosamine, N-acetyl-d-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-d-galactose, organized into trisaccharide repeating units having the sequence --&gt;3)-alpha-d-Fuc4NAc-(1--&gt;4)-beta-d-ManNAcA-(1--&gt;4)-alpha-d-GlcNAc-(1--&gt;. While the precise function of ECA is unknown, it has been linked to the resistance of Shiga-toxin-producing Escherichia coli (STEC) O157:H7 to organic acids and the resistance of Salmonella enterica to bile salts. The final step in the synthesis of 4-acetamido-4,6-dideoxy-d-galactose, the acetyl-coenzyme A (CoA)-dependent acetylation of the 4-amino group, is carried out by TDP-fucosamine acetyltransferase (WecD). We have determined the crystal structure of WecD in apo form at a 1.95-Angstrom resolution and bound to acetyl-CoA at a 1.66-Angstrom resolution. WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars. The crystal structure of WecD, however, represents the first structure of a GNAT family member that acts on nucleotide sugars. Based on this cocrystal structure, we have used flexible docking to generate a WecD-bound model of the acetyl-CoA-TDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. Our structural data show that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of coenzyme A.
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{{STRUCTURE_2ft0| PDB=2ft0 | SCENE= }}
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Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis.,Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A J Bacteriol. 2006 Aug;188(15):5606-17. PMID:16855251<ref>PMID:16855251</ref>
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===Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16855251}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2ft0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_cft073 Escherichia coli cft073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FT0 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:016855251</ref><references group="xtra"/>
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[[Category: Escherichia coli cft073]]
[[Category: Escherichia coli cft073]]
[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]

Revision as of 08:38, 30 September 2014

Crystal structure of TDP-fucosamine acetyltransferase (WecD)- complex with acetyl-CoA

2ft0, resolution 1.66Å

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