1i25
From Proteopedia
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| - | [[Image:1i25.jpg|left|200px]] | + | [[Image:1i25.jpg|left|200px]] |
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| - | '''Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR''' | + | {{Structure |
| + | |PDB= 1i25 |SIZE=350|CAPTION= <scene name='initialview01'>1i25</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I25 is a [ | + | 1I25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I25 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:[http:// | + | The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11790834 11790834] |
[[Category: Ornithoctonus huwena]] | [[Category: Ornithoctonus huwena]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Lu, S Y.]] | [[Category: Lu, S Y.]] | ||
[[Category: Shu, Q.]] | [[Category: Shu, Q.]] | ||
| - | [[Category: disulfide | + | [[Category: disulfide bond]] |
[[Category: insecticidal toxin]] | [[Category: insecticidal toxin]] | ||
[[Category: neurotoxin]] | [[Category: neurotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:44:31 2008'' |
Revision as of 09:44, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR
Overview
The three-dimensional structure of huwentoxin-II (HWTX-II), an insecticidal peptide purified from the venom of spider Selenocosmia huwena with a unique disulfide bond linkage as I-III, II-V, and IV-VI, has been determined using 2D (1)H-NMR. The resulting structure of HWTX-II contains two beta-turns (C4-S7 and K24-W27) and a double-stranded antiparallel beta-sheet (W27-C29 and C34-K36). Although the C-terminal double-stranded beta-sheet cross-linked by two disulfide bonds (II-V and IV-VI in HWTX-II, II-V and III-VI in the ICK molecules) is conserved both in HWTX-II and the ICK molecules, the structure of HWTX-II is unexpected absence of the cystine knot because of its unique disulfide linkage. It suggests that HWTX-II adopts a novel scaffold different from the ICK motif that is adopted by all other spider toxin structures elucidated thus far. Furthermore, the structure of HWTX-II, which conforms to the disulfide-directed beta-hairpin (DDH) motif, not only supports the hypothesis that the ICK is a minor elaboration of the more ancestral DDH motif but also suggests that HWTX-II may have evolved from the same structural ancestor.
About this Structure
1I25 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.
Reference
The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:11790834
Page seeded by OCA on Thu Mar 20 11:44:31 2008
