1i3p
From Proteopedia
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| - | [[Image:1i3p.gif|left|200px]] | + | [[Image:1i3p.gif|left|200px]] |
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| - | '''THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE''' | + | {{Structure |
| + | |PDB= 1i3p |SIZE=350|CAPTION= <scene name='initialview01'>1i3p</scene>, resolution 3.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= P35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46015 Autographa californica nucleopolyhedrovirus]) | ||
| + | }} | ||
| + | |||
| + | '''THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I3P is a [ | + | 1I3P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3P OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of baculovirus P35 reveals a novel conformational change in the reactive site loop after caspase cleavage., dela Cruz WP, Friesen PD, Fisher AJ, J Biol Chem. 2001 Aug 31;276(35):32933-9. Epub 2001 Jun 11. PMID:[http:// | + | Crystal structure of baculovirus P35 reveals a novel conformational change in the reactive site loop after caspase cleavage., dela Cruz WP, Friesen PD, Fisher AJ, J Biol Chem. 2001 Aug 31;276(35):32933-9. Epub 2001 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11402050 11402050] |
[[Category: Autographa californica nucleopolyhedrovirus]] | [[Category: Autographa californica nucleopolyhedrovirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: reactive site loop]] | [[Category: reactive site loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:08 2008'' |
Revision as of 09:45, 20 March 2008
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| , resolution 3.10Å | |||||||
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| Gene: | P35 (Autographa californica nucleopolyhedrovirus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE
Overview
Baculovirus P35 is a universal suppressor of apoptosis that stoichiometrically inhibits cellular caspases in a novel cleavage-dependent mechanism. Upon caspase cleavage at Asp-87, the 10- and 25-kDa cleavage products of P35 remain tightly associated with the inhibited caspase. Mutations in the alpha-helix of the reactive site loop preceding the cleavage site abrogate caspase inhibition and antiapoptotic activity. Substitution of Pro for Val-71, which is located in the middle of this alpha-helix, produces a protein that is cleaved at the requisite Asp-87 but does not remain bound to the caspase. This loss-of-function mutation provided the opportunity to structurally analyze the conformational changes of the P35 reactive site loop after caspase cleavage. We report here the 2.7 A resolution crystal structure of V71P-mutated P35 after cleavage by human caspase-3. The structure reveals a large movement in the carboxyl-terminal side of the reactive site loop that swings down and forms a new beta-strand that augments an existing beta-sheet. Additionally, the hydrophobic amino terminus releases and extends away from the protein core. Similar movements occur when P35 forms an inhibitory complex with human caspase-8. These findings suggest that the alpha-helix mutation may alter the sequential steps or kinetics of the conformational changes required for inhibition, thereby causing P35 loss of function.
About this Structure
1I3P is a Single protein structure of sequence from Autographa californica nucleopolyhedrovirus. Full crystallographic information is available from OCA.
Reference
Crystal structure of baculovirus P35 reveals a novel conformational change in the reactive site loop after caspase cleavage., dela Cruz WP, Friesen PD, Fisher AJ, J Biol Chem. 2001 Aug 31;276(35):32933-9. Epub 2001 Jun 11. PMID:11402050
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