1i3q
From Proteopedia
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| - | [[Image:1i3q.gif|left|200px]] | + | [[Image:1i3q.gif|left|200px]] |
| - | + | ||
| - | '''RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION''' | + | {{Structure |
| + | |PDB= 1i3q |SIZE=350|CAPTION= <scene name='initialview01'>1i3q</scene>, resolution 3.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I3Q is a [ | + | 1I3Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry 1EN0. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3Q OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:[http:// | + | Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11313498 11313498] |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: multiprotein complex]] | [[Category: multiprotein complex]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | [[Category: zinc | + | [[Category: zinc motif]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:08 2008'' |
Revision as of 09:45, 20 March 2008
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| , resolution 3.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION
Overview
Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
About this Structure
1I3Q is a Protein complex structure of sequences from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 1EN0. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:11313498
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