1i4k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1i4k.gif|left|200px]]<br /><applet load="1i4k" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1i4k.gif|left|200px]]
-
caption="1i4k, resolution 2.5&Aring;" />
+
 
-
'''CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION'''<br />
+
{{Structure
 +
|PDB= 1i4k |SIZE=350|CAPTION= <scene name='initialview01'>1i4k</scene>, resolution 2.5&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene>
 +
|ACTIVITY=
 +
|GENE= AF0875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
 +
}}
 +
 
 +
'''CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1I4K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4K OCA].
+
1I4K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4K OCA].
==Reference==
==Reference==
-
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex., Toro I, Thore S, Mayer C, Basquin J, Seraphin B, Suck D, EMBO J. 2001 May 1;20(9):2293-303. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11331594 11331594]
+
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex., Toro I, Thore S, Mayer C, Basquin J, Seraphin B, Suck D, EMBO J. 2001 May 1;20(9):2293-303. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11331594 11331594]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 25: Line 34:
[[Category: snrnp]]
[[Category: snrnp]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:01 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:28 2008''

Revision as of 09:45, 20 March 2008

Image:1i4k.gif


PDB ID 1i4k

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands:
Gene: AF0875 (Archaeoglobus fulgidus)
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION


Overview

Eukaryotic Sm and Sm-like proteins associate with RNA to form the core domain of ribonucleoprotein particles involved in pre-mRNA splicing and other processes. Recently, putative Sm proteins of unknown function have been identified in ARCHAEA: We show by immunoprecipitation experiments that the two Sm proteins present in Archaeoglobus fulgidus (AF-Sm1 and AF-Sm2) associate with RNase P RNA in vivo, suggesting a role in tRNA processing. The AF-Sm1 protein also interacts specifically with oligouridylate in vitro. We have solved the crystal structures of this protein and a complex with RNA. AF-Sm1 forms a seven-membered ring, with the RNA interacting inside the central cavity on one face of the doughnut-shaped complex. The bases are bound via stacking and specific hydrogen bonding contacts in pockets lined by residues highly conserved in archaeal and eukaryotic Sm proteins, while the phosphates remain solvent accessible. A comparison with the structures of human Sm protein dimers reveals closely related monomer folds and intersubunit contacts, indicating that the architecture of the Sm core domain and RNA binding have been conserved during evolution.

About this Structure

1I4K is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex., Toro I, Thore S, Mayer C, Basquin J, Seraphin B, Suck D, EMBO J. 2001 May 1;20(9):2293-303. PMID:11331594

Page seeded by OCA on Thu Mar 20 11:45:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i4k"
Personal tools