1i5j
From Proteopedia
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| - | [[Image:1i5j.gif|left|200px]] | + | [[Image:1i5j.gif|left|200px]] |
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| - | '''NMR STRUCTURE OF HUMAN APOLIPOPROTEIN C-II IN THE PRESENCE OF SDS''' | + | {{Structure |
| + | |PDB= 1i5j |SIZE=350|CAPTION= <scene name='initialview01'>1i5j</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR STRUCTURE OF HUMAN APOLIPOPROTEIN C-II IN THE PRESENCE OF SDS''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I5J is a [ | + | 1I5J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5J OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate., MacRaild CA, Hatters DM, Howlett GJ, Gooley PR, Biochemistry. 2001 May 8;40(18):5414-21. PMID:[http:// | + | NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate., MacRaild CA, Hatters DM, Howlett GJ, Gooley PR, Biochemistry. 2001 May 8;40(18):5414-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11331005 11331005] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-lipid interaction]] | [[Category: protein-lipid interaction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:49 2008'' |
Revision as of 09:45, 20 March 2008
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NMR STRUCTURE OF HUMAN APOLIPOPROTEIN C-II IN THE PRESENCE OF SDS
Contents |
Overview
The structure and protein-detergent interactions of apolipoprotein C-II (apoC-II) in the presence of SDS micelles have been investigated using circular dichroism and heteronuclear NMR techniques applied to (15)N-labeled protein. Micellar SDS, a commonly used mimetic of the lipoprotein surface, inhibits the aggregation of apoC-II and induces a stable structure containing approximately 60% alpha-helix as determined by circular dichroism. NMR reveals the first 12 residues of apoC-II to be structurally heterogeneous and largely disordered, with the rest of the protein forming a predominantly helical structure. Three regions of helical conformation, residues 16-36, 50-56, and 63-77, are well-defined by NMR-derived constraints, with the intervening regions showing more loosely defined helical conformation. The structure of apoC-II is compared to that determined for other apolipoproteins in a similar environment. Our results shed light on the lipid interactions of apoC-II and its mechanism of lipoprotein lipase activation.
Disease
Known disease associated with this structure: Hyperlipoproteinemia, type Ib OMIM:[608083]
About this Structure
1I5J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate., MacRaild CA, Hatters DM, Howlett GJ, Gooley PR, Biochemistry. 2001 May 8;40(18):5414-21. PMID:11331005
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