1i7h
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1i7h.gif|left|200px]] | + | [[Image:1i7h.gif|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STURCUTURE OF FDX''' | + | {{Structure |
| + | |PDB= 1i7h |SIZE=350|CAPTION= <scene name='initialview01'>1i7h</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STURCUTURE OF FDX''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1I7H is a [ | + | 1I7H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7H OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters., Kakuta Y, Horio T, Takahashi Y, Fukuyama K, Biochemistry. 2001 Sep 18;40(37):11007-12. PMID:[http:// | + | Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters., Kakuta Y, Horio T, Takahashi Y, Fukuyama K, Biochemistry. 2001 Sep 18;40(37):11007-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11551196 11551196] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: Takahashi, Y.]] | [[Category: Takahashi, Y.]] | ||
[[Category: FES]] | [[Category: FES]] | ||
| - | [[Category: 2fe- | + | [[Category: 2fe-2]] |
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:46:31 2008'' |
Revision as of 09:46, 20 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STURCUTURE OF FDX
Overview
Escherichia coli ferredoxin (Fdx) is an adrenodoxin-type [2Fe-2S] ferredoxin. Recent genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins. Fdx probably functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters. Its crystal structure was determined by the multiple-wavelength anomalous dispersion method using the iron atoms in the [2Fe-2S] cluster of the protein and then refined to R and R(free) values of 0.255 and 0.278, respectively, at 1.7 A resolution. The structure of Fdx is similar to the structures of bovine adrenodoxin (Adx) and Pseudomonas putida putidaredoxin (Pdx) whose respective root-mean-square deviations of the corresponding Calpha atoms are 1.8 and 2.2 A. This analysis also revealed the structure of the C-terminal residues protruding into the solvent, which is missing in Adx and Pdx. The [2Fe-2S] cluster is located at the edge of the molecule and bonds with the Sgamma atoms of Cys42, Cys48, Cys51, and Cys87. Electrostatic potential analysis showed that the surface of Fdx has two negatively charged areas separated by a hydrophobic lane. One is conserved on the surface of Adx which is an area of interaction with adrenodoxin reductase. Cys46 is located on the molecular surface in the vicinity of the [2Fe-2S] cluster, an indication that it may be involved in Fe-S cluster formation.
About this Structure
1I7H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters., Kakuta Y, Horio T, Takahashi Y, Fukuyama K, Biochemistry. 2001 Sep 18;40(37):11007-12. PMID:11551196
Page seeded by OCA on Thu Mar 20 11:46:31 2008
