1i7z
From Proteopedia
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| - | [[Image:1i7z.jpg|left|200px]] | + | [[Image:1i7z.jpg|left|200px]] |
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| - | '''ANTIBODY GNC92H2 BOUND TO LIGAND''' | + | {{Structure |
| + | |PDB= 1i7z |SIZE=350|CAPTION= <scene name='initialview01'>1i7z</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=COC:COCAINE'>COC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''ANTIBODY GNC92H2 BOUND TO LIGAND''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I7Z is a [ | + | 1I7Z is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_and_homo_sapiens Mus musculus and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7Z OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a cocaine-binding antibody., Larsen NA, Zhou B, Heine A, Wirsching P, Janda KD, Wilson IA, J Mol Biol. 2001 Aug 3;311(1):9-15. PMID:[http:// | + | Crystal structure of a cocaine-binding antibody., Larsen NA, Zhou B, Heine A, Wirsching P, Janda KD, Wilson IA, J Mol Biol. 2001 Aug 3;311(1):9-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11469854 11469854] |
[[Category: Mus musculus and homo sapiens]] | [[Category: Mus musculus and homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:46:46 2008'' |
Revision as of 09:46, 20 March 2008
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| , resolution 2.3Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
ANTIBODY GNC92H2 BOUND TO LIGAND
Contents |
Overview
Murine monoclonal antibody GNC92H2 was elicited by active immunization with a cocaine immunoconjugate and binds free cocaine with excellent specificity and moderate affinity. Improvement of affinity, as well as humanization of GNC92H2, would be advantageous in immunopharmacotherapy for cocaine addiction, and for emergency cases of drug overdose. Toward this end, the crystal structure of an engineered murine-human chimeric Fab of GNC92H2 complexed with cocaine was determined at 2.3 A resolution. Structural analysis reveals a binding pocket with high shape and charge complementarity to the cocaine framework, which explains the specificity for cocaine, as opposed to the pharmacologically inactive cocaine metabolites. Importantly, the structure provides a foundation for mutagenesis to enhance the binding affinity for cocaine and potent cocaine derivatives, such as cocaethylene, and for additional humanization of the antibody.
Disease
Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]
About this Structure
1I7Z is a Protein complex structure of sequences from Mus musculus and homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a cocaine-binding antibody., Larsen NA, Zhou B, Heine A, Wirsching P, Janda KD, Wilson IA, J Mol Biol. 2001 Aug 3;311(1):9-15. PMID:11469854
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