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2gfi

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[[Image:2gfi.png|left|200px]]
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==Crystal structure of the phytase from D. castellii at 2.3 A==
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<StructureSection load='2gfi' size='340' side='right' caption='[[2gfi]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schwanniomyces_capriottii Schwanniomyces capriottii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GFI FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gfi RCSB], [http://www.ebi.ac.uk/pdbsum/2gfi PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gfi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.
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{{STRUCTURE_2gfi| PDB=2gfi | SCENE= }}
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Structure of Debaryomyces castellii CBS 2923 phytase.,Ragon M, Hoh F, Aumelas A, Chiche L, Moulin G, Boze H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt, 4):321-6. Epub 2009 Mar 25. PMID:19342770<ref>PMID:19342770</ref>
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===Crystal structure of the phytase from D. castellii at 2.3 A===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19342770}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2gfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schwanniomyces_capriottii Schwanniomyces capriottii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019342770</ref><references group="xtra"/>
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[[Category: 3-phytase]]
[[Category: 3-phytase]]
[[Category: Schwanniomyces capriottii]]
[[Category: Schwanniomyces capriottii]]
[[Category: Hoh, F.]]
[[Category: Hoh, F.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 11:00, 30 September 2014

Crystal structure of the phytase from D. castellii at 2.3 A

2gfi, resolution 2.29Å

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