1i8n
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1i8n.jpg|left|200px]] | + | [[Image:1i8n.jpg|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF LEECH ANTI-PLATELET PROTEIN''' | + | {{Structure |
| + | |PDB= 1i8n |SIZE=350|CAPTION= <scene name='initialview01'>1i8n</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ROP:PROPIONAMIDE'>ROP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= LAPP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6410 Haementeria officinalis]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF LEECH ANTI-PLATELET PROTEIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1I8N is a [ | + | 1I8N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haementeria_officinalis Haementeria officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8N OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of leech anti-platelet protein, an inhibitor of haemostasis., Huizinga EG, Schouten A, Connolly TM, Kroon J, Sixma JJ, Gros P, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1071-8. Epub 2001, Jul 23. PMID:[http:// | + | The structure of leech anti-platelet protein, an inhibitor of haemostasis., Huizinga EG, Schouten A, Connolly TM, Kroon J, Sixma JJ, Gros P, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1071-8. Epub 2001, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11468390 11468390] |
[[Category: Haementeria officinalis]] | [[Category: Haementeria officinalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: pan module]] | [[Category: pan module]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:00 2008'' |
Revision as of 09:47, 20 March 2008
| |||||||
| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | LAPP (Haementeria officinalis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF LEECH ANTI-PLATELET PROTEIN
Overview
Leech anti-platelet protein (LAPP) from the leech Haementeria officinalis is a collagen-binding protein that inhibits the collagen-mediated adhesion of blood platelets. The crystal structure of recombinant LAPP has been determined using single isomorphous replacement with anomalous scattering combined with solvent flattening and threefold molecular averaging. The model of LAPP has been refined to 2.2 A resolution (R factor 21.5%; free R factor 24.0%). LAPP contains an 89-residue C-terminal domain consisting of a central six-stranded antiparallel beta-sheet flanked on one side by an alpha-helix and on the other side by two extended loops with little secondary structure. A 36-residue N-terminal region is not visible in the electron-density map. This region is rich in glycine and lacks hydrophobic residues. It probably does not have a compact globular fold, but instead has an extended conformation and is flexible. The crystal packing suggests that LAPP may form tightly interacting dimers. The fold of the C-terminal domain of LAPP closely resembles that of the N-domain of hepatocyte growth factor (HGF), which classifies LAPP as a PAN domain. However, no significant sequence homology exists between LAPP and other PAN domains. Common structural features between LAPP and the HGF N-domain include two disulfide bonds that link the alpha-helix to the central region of the protein and five residues with a conserved hydrophobic nature that are located in the core of the domain. These conserved structural features may be an important determinant of the PAN-domain type of fold.
About this Structure
1I8N is a Single protein structure of sequence from Haementeria officinalis. Full crystallographic information is available from OCA.
Reference
The structure of leech anti-platelet protein, an inhibitor of haemostasis., Huizinga EG, Schouten A, Connolly TM, Kroon J, Sixma JJ, Gros P, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1071-8. Epub 2001, Jul 23. PMID:11468390
Page seeded by OCA on Thu Mar 20 11:47:00 2008
