1ia6

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[[Image:1ia6.gif|left|200px]]<br /><applet load="1ia6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ia6.gif|left|200px]]
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caption="1ia6, resolution 1.80&Aring;" />
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'''CRYSTAL STRUCTURE OF THE CELLULASE CEL9M OF C. CELLULOLYTICUM'''<br />
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{{Structure
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|PDB= 1ia6 |SIZE=350|CAPTION= <scene name='initialview01'>1ia6</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF THE CELLULASE CEL9M OF C. CELLULOLYTICUM'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1IA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IA6 OCA].
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1IA6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IA6 OCA].
==Reference==
==Reference==
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Crystal structure of the cellulase Cel9M enlightens structure/function relationships of the variable catalytic modules in glycoside hydrolases., Parsiegla G, Belaich A, Belaich JP, Haser R, Biochemistry. 2002 Sep 17;41(37):11134-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12220178 12220178]
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Crystal structure of the cellulase Cel9M enlightens structure/function relationships of the variable catalytic modules in glycoside hydrolases., Parsiegla G, Belaich A, Belaich JP, Haser R, Biochemistry. 2002 Sep 17;41(37):11134-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12220178 12220178]
[[Category: Cellulase]]
[[Category: Cellulase]]
[[Category: Clostridium cellulolyticum]]
[[Category: Clostridium cellulolyticum]]
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[[Category: cellullase]]
[[Category: cellullase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:38 2008''

Revision as of 09:47, 20 March 2008

Image:1ia6.gif


PDB ID 1ia6

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , , and
Activity: Cellulase, with EC number 3.2.1.4
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE CELLULASE CEL9M OF C. CELLULOLYTICUM


Overview

Cellulases cleave the beta-1.4 glycosidic bond of cellulose. They have been characterized as endo or exo and processive or nonprocessive cellulases according to their action mode on the substrate. Different types of these cellulases may coexist in the same glycoside hydrolase family, which have been classified according to their sequence homology and catalytic mechanism. The bacterium C. celluloyticum produces a set of different cellulases who belong mostly to glycoside hydrolase families 5 and 9. As an adaptation of the organism to different macroscopic substrates organizations and to maximize its cooperative digestion, it is expected that cellulases of these families are active on the various macroscopic organizations of cellulose chains. The nonprocessive cellulase Cel9M is the shortest variant of family 9 cellulases (subgroup 9(C)) which contains only the catalytic module to interact with the substrate. The crystal structures of free native Cel9M and its complex with cellobiose have been solved to 1.8 and 2.0 A resolution, respectively. Other structurally known family 9 cellulases are the nonprocessive endo-cellulase Cel9D from C. thermocellum and the processive endo-cellulase Cel9A from T. fusca, from subgroups 9(B1) and 9(A), respectively, whose catalytic modules are fused to a second domain. These enzymes differ in their activity on substrates with specific macroscopic appearances. The comparison of the catalytic module of Cel9M with the two other known GH family 9 structures may give clues to explain its substrate profile and action mode.

About this Structure

1IA6 is a Single protein structure of sequence from Clostridium cellulolyticum. Full crystallographic information is available from OCA.

Reference

Crystal structure of the cellulase Cel9M enlightens structure/function relationships of the variable catalytic modules in glycoside hydrolases., Parsiegla G, Belaich A, Belaich JP, Haser R, Biochemistry. 2002 Sep 17;41(37):11134-42. PMID:12220178

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