2o2c

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{{STRUCTURE_2o2c| PDB=2o2c | SCENE= }}
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==Crystal structure of phosphoglucose isomerase from T. brucei containing glucose-6-phosphate in the active site==
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===Crystal structure of phosphoglucose isomerase from T. brucei containing glucose-6-phosphate in the active site===
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<StructureSection load='2o2c' size='340' side='right' caption='[[2o2c]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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{{ABSTRACT_PUBMED_18561188}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o2c]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O2C FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G6Q:GLUCOSE-6-PHOSPHATE'>G6Q</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o2d|2o2d]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PGI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 Trypanosoma brucei brucei])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o2c RCSB], [http://www.ebi.ac.uk/pdbsum/2o2c PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o2/2o2c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enzymes of glycolysis in Trypanosoma brucei have been identified as potential drug targets for African sleeping sickness because glycolysis is the only source of ATP for the bloodstream form of this parasite. Several inhibitors were previously reported to bind preferentially to trypanosomal phosphoglucose isomerase (PGI, the second enzyme in glycolysis) than to mammalian PGIs, which suggests that PGI might make a good target for species-specific drug design. Herein, we report recombinant expression, purification, crystallization and X-ray crystal structure determination of T. brucei PGI. One structure solved at 1.6 A resolution contains a substrate, D-glucose-6-phosphate, in an extended conformation in the active site. A second structure solved at 1.9 A resolution contains a citrate molecule in the active site. The structures are compared with the crystal structures of PGI from humans and from Leishmania mexicana. The availability of recombinant tPGI and its first high-resolution crystal structures are initial steps in considering this enzyme as a potential drug target. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
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==About this Structure==
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Crystal structure of phosphoglucose isomerase from Trypanosoma brucei complexed with glucose-6-phosphate at 1.6 A resolution.,Arsenieva D, Appavu BL, Mazock GH, Jeffery CJ Proteins. 2008 Jun 16. PMID:18561188<ref>PMID:18561188</ref>
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[[2o2c]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O2C OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[Phosphoglucoisomerase|Phosphoglucoisomerase]]
*[[Phosphoglucoisomerase|Phosphoglucoisomerase]]
*[[Phosphoglucose isomerase|Phosphoglucose isomerase]]
*[[Phosphoglucose isomerase|Phosphoglucose isomerase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018561188</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Glucose-6-phosphate isomerase]]
[[Category: Glucose-6-phosphate isomerase]]
[[Category: Trypanosoma brucei brucei]]
[[Category: Trypanosoma brucei brucei]]

Revision as of 18:47, 30 September 2014

Crystal structure of phosphoglucose isomerase from T. brucei containing glucose-6-phosphate in the active site

2o2c, resolution 1.58Å

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