1ibx
From Proteopedia
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| - | [[Image:1ibx.gif|left|200px]] | + | [[Image:1ibx.gif|left|200px]] |
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| - | '''NMR STRUCTURE OF DFF40 AND DFF45 N-TERMINAL DOMAIN COMPLEX''' | + | {{Structure |
| + | |PDB= 1ibx |SIZE=350|CAPTION= <scene name='initialview01'>1ibx</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= DFF40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), DFF45 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= STREPTOCOCCUS SP. AND HOMO SAPIENS]) | ||
| + | }} | ||
| + | |||
| + | '''NMR STRUCTURE OF DFF40 AND DFF45 N-TERMINAL DOMAIN COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IBX is a [ | + | 1IBX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Streptococcus_sp._and_homo_sapiens Streptococcus sp. and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBX OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45., Zhou P, Lugovskoy AA, McCarty JS, Li P, Wagner G, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6051-5. PMID:[http:// | + | Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45., Zhou P, Lugovskoy AA, McCarty JS, Li P, Wagner G, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6051-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11371636 11371636] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:48:16 2008'' |
Revision as of 09:48, 20 March 2008
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| Gene: | DFF40 (Homo sapiens), DFF45 (STREPTOCOCCUS SP. AND HOMO SAPIENS) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR STRUCTURE OF DFF40 AND DFF45 N-TERMINAL DOMAIN COMPLEX
Contents |
Overview
Apoptotic DNA fragmentation is mediated by a caspase-activated DNA fragmentation factor (DFF)40. Expression and folding of DFF40 require the presence of DFF45, which also acts as a nuclease inhibitor before DFF40 activation by execution caspases. The N-terminal domains (NTDs) of both proteins are homologous, and their interaction plays a key role in the proper functioning of this two-component system. Here we report that the NTD of DFF45 alone is unstructured in solution, and its folding is induced upon binding to DFF40 NTD. Therefore, folding of both proteins regulates the formation of the DFF40/DFF45 complex. The solution structure of the heterodimeric complex between NTDs of DFF40 and DFF45 reported here shows that the mutual chaperoning includes the formation of an extensive network of intermolecular interactions that bury a hydrophobic cluster inside the interface, surrounded by intermolecular salt bridges.
Disease
Known disease associated with this structure: Cerebral arteriopathy with subcortical infarcts and leukoencephalopathy OMIM:[600276]
About this Structure
1IBX is a Protein complex structure of sequences from Homo sapiens and Streptococcus sp. and homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45., Zhou P, Lugovskoy AA, McCarty JS, Li P, Wagner G, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6051-5. PMID:11371636
Page seeded by OCA on Thu Mar 20 11:48:16 2008
