2rh1

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{{STRUCTURE_2rh1| PDB=2rh1 | SCENE= }}
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==High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.==
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===High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.===
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<StructureSection load='2rh1' size='340' side='right' caption='[[2rh1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_17962520}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. The April 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Adrenergic Receptors'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_4 10.2210/rcsb_pdb/mom_2008_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RH1 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ACM:ACETAMIDE'>ACM</scene>, <scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CAU:(2S)-1-(9H-CARBAZOL-4-YLOXY)-3-(ISOPROPYLAMINO)PROPAN-2-OL'>CAU</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADRB2, ADRB2R, B2AR / E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rh1 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh1 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/2rh1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors constitute the largest family of eukaryotic signal transduction proteins that communicate across the membrane. We report the crystal structure of a human beta2-adrenergic receptor-T4 lysozyme fusion protein bound to the partial inverse agonist carazolol at 2.4 angstrom resolution. The structure provides a high-resolution view of a human G protein-coupled receptor bound to a diffusible ligand. Ligand-binding site accessibility is enabled by the second extracellular loop, which is held out of the binding cavity by a pair of closely spaced disulfide bridges and a short helical segment within the loop. Cholesterol, a necessary component for crystallization, mediates an intriguing parallel association of receptor molecules in the crystal lattice. Although the location of carazolol in the beta2-adrenergic receptor is very similar to that of retinal in rhodopsin, structural differences in the ligand-binding site and other regions highlight the challenges in using rhodopsin as a template model for this large receptor family.
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==About this Structure==
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High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.,Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SG, Thian FS, Kobilka TS, Choi HJ, Kuhn P, Weis WI, Kobilka BK, Stevens RC Science. 2007 Nov 23;318(5854):1258-65. Epub 2007 Oct 25. PMID:17962520<ref>PMID:17962520</ref>
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[[2rh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. The April 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Adrenergic Receptors'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_4 10.2210/rcsb_pdb/mom_2008_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH1 OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
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*[[Beta-2 Adrenergic Receptor|Beta-2 Adrenergic Receptor]]
*[[Beta-2 Adrenergic Receptor|Beta-2 Adrenergic Receptor]]
*[[G protein-coupled receptor|G protein-coupled receptor]]
*[[G protein-coupled receptor|G protein-coupled receptor]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
*[[Nobel Prizes for 3D Molecular Structure|Nobel Prizes for 3D Molecular Structure]]
*[[Nobel Prizes for 3D Molecular Structure|Nobel Prizes for 3D Molecular Structure]]
*[[Suggestions for new articles|Suggestions for new articles]]
*[[Suggestions for new articles|Suggestions for new articles]]
*[[User:Wayne Decatur/NASCE2011|User:Wayne Decatur/NASCE2011]]
*[[User:Wayne Decatur/NASCE2011|User:Wayne Decatur/NASCE2011]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017962520</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Adrenergic Receptors]]
[[Category: Adrenergic Receptors]]
[[Category: Enterobacteria phage t4]]
[[Category: Enterobacteria phage t4]]

Revision as of 20:11, 30 September 2014

High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.

2rh1, resolution 2.40Å

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