2v0s

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[[Image:2v0s.png|left|200px]]
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==CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LR1) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE==
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<StructureSection load='2v0s' size='340' side='right' caption='[[2v0s]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2v0s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V0S FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1vyb|1vyb]], [[2v0r|2v0r]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v0s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v0s RCSB], [http://www.ebi.ac.uk/pdbsum/2v0s PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v0/2v0s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of new L1 and Alu elements that presently account for approximately 28% of the human genome. L1-EN bears considerable technological interest, because its target selectivity may ultimately be engineered to allow the site-specific integration of DNA into defined genomic locations. Based on the crystal structure, we generated L1-EN mutants to analyze and manipulate DNA target site recognition. Crystal structures and their dynamic and functional analysis show entire loop grafts to be feasible, resulting in altered specificity, while individual point mutations do not change the nicking pattern of L1-EN. Structural parameters of the DNA target seem more important for recognition than the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro are less well defined than the respective integration site consensus in vivo. This suggests that additional factors other than the DNA nicking specificity of L1-EN contribute to the targeted integration of non-LTR retrotransposons.
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{{STRUCTURE_2v0s| PDB=2v0s | SCENE= }}
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Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.,Repanas K, Zingler N, Layer LE, Schumann GG, Perrakis A, Weichenrieder O Nucleic Acids Res. 2007;35(14):4914-26. Epub 2007 Jul 10. PMID:17626046<ref>PMID:17626046</ref>
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===CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LR1) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17626046}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2v0s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0S OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:017626046</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Layer, L E.]]
[[Category: Layer, L E.]]

Revision as of 01:29, 1 October 2014

CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LR1) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE

2v0s, resolution 1.80Å

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