2v6f

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2v6f" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2v6f.png|left|200px]]
+
==STRUCTURE OF PROGESTERONE 5BETA-REDUCTASE FROM DIGITALIS LANATA==
 +
<StructureSection load='2v6f' size='340' side='right' caption='[[2v6f]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2v6f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Digitalis_lanata Digitalis lanata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V6F FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v6g|2v6g]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v6f RCSB], [http://www.ebi.ac.uk/pdbsum/2v6f PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v6/2v6f_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Progesterone 5beta-reductase (5beta-POR) catalyzes the stereospecific reduction of progesterone to 5beta-pregnane-3,20-dione and is a key enzyme in the biosynthetic pathway of cardenolides in Digitalis (foxglove) plants. Sequence considerations suggested that 5beta-POR is a member of the short chain dehydrogenase/reductase (SDR) family of proteins but at the same time revealed that the sequence motifs that in standard SDRs contain the catalytically important residues are missing. Here we present crystal structures of 5beta-POR from Digitalis lanata in complex with NADP(+) at 2.3A and without cofactor bound at 2.4A resolution together with a model of a ternary complex consisting of 5beta-POR, NADP(+), and progesterone. Indeed, 5beta-POR displays the fold of an extended SDR. The architecture of the active site is, however, unprecedented because none of the standard catalytic residues are structurally conserved. A tyrosine (Tyr-179) and a lysine residue (Lys-147) are present in the active site, but they are displayed from novel positions and are part of novel sequence motifs. Mutating Tyr-179 to either alanine or phenylalanine completely abolishes the enzymatic activity. We propose that the distinct topology reflects the fact that 5beta-POR reduces a conjugated double bond in a steroid substrate via a 1-4 addition mechanism and that this requires a repositioning of the catalytically important residues. Our observation that the sequence motifs that line the active site are conserved in a number of bacterial and plant enzymes of yet unknown function leads us to the proposition that 5beta-POR defines a novel class of SDRs.
-
{{STRUCTURE_2v6f| PDB=2v6f | SCENE= }}
+
The crystal structure of progesterone 5beta-reductase from Digitalis lanata defines a novel class of short chain dehydrogenases/reductases.,Thorn A, Egerer-Sieber C, Jager CM, Herl V, Muller-Uri F, Kreis W, Muller YA J Biol Chem. 2008 Jun 20;283(25):17260-9. Epub 2007 Nov 21. PMID:18032383<ref>PMID:18032383</ref>
-
===STRUCTURE OF PROGESTERONE 5BETA-REDUCTASE FROM DIGITALIS LANATA===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_18032383}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2v6f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Digitalis_lanata Digitalis lanata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6F OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:018032383</ref><references group="xtra"/>
+
[[Category: Digitalis lanata]]
[[Category: Digitalis lanata]]
[[Category: Egerer-Sieber, C.]]
[[Category: Egerer-Sieber, C.]]

Revision as of 01:54, 1 October 2014

STRUCTURE OF PROGESTERONE 5BETA-REDUCTASE FROM DIGITALIS LANATA

2v6f, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v6f"
Views
Personal tools
Navigation
Toolbox