2wn5

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[[Image:2wn5.png|left|200px]]
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==STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION IN THE ENZYMATIC COMPONENT OF ADP-RIBOSYLTRANSFERASE TOXIN CDTA FROM CLOSTRIDIUM DIFFICILE==
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<StructureSection load='2wn5' size='340' side='right' caption='[[2wn5]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wn5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WN5 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wn6|2wn6]], [[2wn8|2wn8]], [[2wn7|2wn7]], [[2wn4|2wn4]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wn5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wn5 RCSB], [http://www.ebi.ac.uk/pdbsum/2wn5 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/2wn5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ADP-ribosylation is one of the favored modes of cell intoxication employed by several bacteria. Clostridium difficile is recognized to be an important nosocomial pathogen associated with considerable morbidity and attributable mortality. Along with its two well known toxins, Toxin A and Toxin B, it produces an ADP-ribosylating toxin that targets monomeric actin of the target cell. Like other Clostridial actin ADP-ribosylating toxins, this binary toxin, known as C. difficile toxin (CDT), is composed of two subunits, CDTa and CDTb. In this study, we present high resolution crystal structures of CDTa in its native form (at pH 4.0, 8.5, and 9.0) and in complex with ADP-ribose donors, NAD and NADPH (at pH 9.0). The crystal structures of the native protein show "pronounced conformational flexibility" confined to the active site region of the protein and "enhanced" disorder at low pH, whereas the complex structures highlight significant differences in "ligand specificity" compared with the enzymatic subunit of a close homologue, Clostridium perfringens iota toxin. Specifically in CDTa, two of the suggested catalytically important residues (Glu-385 and Glu-387) seem to play no role or a less important role in ligand binding. These structural data provide the first detailed information on protein-donor substrate complex stabilization in CDTa, which may have implications in understanding CDT recognition.
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{{STRUCTURE_2wn5| PDB=2wn5 | SCENE= }}
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Structural basis for substrate recognition in the enzymatic component of ADP-ribosyltransferase toxin CDTa from Clostridium difficile.,Sundriyal A, Roberts AK, Shone CC, Acharya KR J Biol Chem. 2009 Oct 16;284(42):28713-9. Epub 2009 Aug 19. PMID:19692332<ref>PMID:19692332</ref>
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===STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION IN THE ENZYMATIC COMPONENT OF ADP-RIBOSYLTRANSFERASE TOXIN CDTA FROM CLOSTRIDIUM DIFFICILE===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19692332}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2wn5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN5 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019692332</ref><references group="xtra"/>
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[[Category: Acharya, K R.]]
[[Category: Acharya, K R.]]
[[Category: Roberts, A K.]]
[[Category: Roberts, A K.]]

Revision as of 02:11, 1 October 2014

STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION IN THE ENZYMATIC COMPONENT OF ADP-RIBOSYLTRANSFERASE TOXIN CDTA FROM CLOSTRIDIUM DIFFICILE

2wn5, resolution 1.90Å

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