1ipe

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[[Image:1ipe.jpg|left|200px]]<br /><applet load="1ipe" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ipe.jpg|left|200px]]
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caption="1ipe, resolution 2.50&Aring;" />
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'''TROPINONE REDUCTASE-II COMPLEXED WITH NADPH'''<br />
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{{Structure
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|PDB= 1ipe |SIZE=350|CAPTION= <scene name='initialview01'>1ipe</scene>, resolution 2.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NDP:NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NDP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236]
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|GENE=
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}}
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'''TROPINONE REDUCTASE-II COMPLEXED WITH NADPH'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1IPE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium] with <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IPE OCA].
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1IPE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IPE OCA].
==Reference==
==Reference==
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Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes., Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H, Biochemistry. 2003 May 20;42(19):5566-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12741812 12741812]
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Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes., Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H, Biochemistry. 2003 May 20;42(19):5566-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12741812 12741812]
[[Category: Datura stramonium]]
[[Category: Datura stramonium]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rsgi]]
[[Category: rsgi]]
[[Category: short-chain dehydrogenase]]
[[Category: short-chain dehydrogenase]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: tropane alkaloid biosynthesis]]
[[Category: tropane alkaloid biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:53:15 2008''

Revision as of 09:53, 20 March 2008

Image:1ipe.jpg


PDB ID 1ipe

Drag the structure with the mouse to rotate
, resolution 2.50Å
Ligands:
Activity: Tropinone reductase II, with EC number 1.1.1.236
Coordinates: save as pdb, mmCIF, xml



TROPINONE REDUCTASE-II COMPLEXED WITH NADPH


Overview

To understand the catalytic mechanism of an enzyme, it is crucial to determine the crystallographic structures corresponding to the individual reaction steps. Here, we report two crystal structures of enzyme-substrate complexes prior to reaction initiation: tropinone reductase-II (TR-II)-NADPH and TR-II-NADPH-tropinone complexes, determined from the identical crystals. A combination of two kinetic crystallographic techniques, a continuous flow of the substrates and Laue diffraction measurements, enabled us to capture the transit structures prior to the reaction proceeding. A structure comparison of the enzyme-substrate complex elucidated in this study with the enzyme-product complex in our previous study indicates that one of the substrates, tropinone, is rotated relative to the product so as to make the spatial organization in the active site favorable for the reaction to proceed. Side chains of the residues in the active site also alter their conformations to keep the complementarity of the space for the substrate or the product and to assist the rotational movement.

About this Structure

1IPE is a Single protein structure of sequence from Datura stramonium. Full crystallographic information is available from OCA.

Reference

Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes., Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H, Biochemistry. 2003 May 20;42(19):5566-73. PMID:12741812

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