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2wwl

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[[Image:2wwl.png|left|200px]]
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==E.COLI 70S RIBOSOME STALLED DURING TRANSLATION OF TNAC LEADER PEPTIDE. THIS FILE CONTAINS THE 30S, THE P-SITE TRNA AND THE TNAC LEADER PEPTIDE (PART 1 OF 2).==
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<StructureSection load='2wwl' size='340' side='right' caption='[[2wwl]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wwl]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WWL FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wwq|2wwq]], [[1s03|1s03]], [[2avy|2avy]], [[2aw7|2aw7]], [[1m5g|1m5g]], [[1mj1|1mj1]], [[1zn1|1zn1]], [[2vho|2vho]], [[1p6g|1p6g]], [[2vhp|2vhp]], [[1p87|1p87]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wwl RCSB], [http://www.ebi.ac.uk/pdbsum/2wwl PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/2wwl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. We determined a 5.8 angstrom-resolution cryo-electron microscopy and single-particle reconstruction of a ribosome stalled during translation of the tnaC leader gene. The nascent chain was extended within the exit tunnel, making contacts with ribosomal components at distinct sites. Upon stalling, two conserved residues within the peptidyltransferase center adopted conformations that preclude binding of release factors. We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel.
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{{STRUCTURE_2wwl| PDB=2wwl | SCENE= }}
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Structural insight into nascent polypeptide chain-mediated translational stalling.,Seidelt B, Innis CA, Wilson DN, Gartmann M, Armache JP, Villa E, Trabuco LG, Becker T, Mielke T, Schulten K, Steitz TA, Beckmann R Science. 2009 Dec 4;326(5958):1412-5. Epub 2009 Oct 29. PMID:19933110<ref>PMID:19933110</ref>
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===E.COLI 70S RIBOSOME STALLED DURING TRANSLATION OF TNAC LEADER PEPTIDE. THIS FILE CONTAINS THE 30S, THE P-SITE TRNA AND THE TNAC LEADER PEPTIDE (PART 1 OF 2).===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19933110}}
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==About this Structure==
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[[2wwl]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWL OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10|Ribosomal protein S10]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein S11|Ribosomal protein S11]]
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== References ==
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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<references/>
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*[[Ribosomal protein S13|Ribosomal protein S13]]
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__TOC__
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*[[Ribosomal protein S14|Ribosomal protein S14]]
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</StructureSection>
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*[[Ribosomal protein S15|Ribosomal protein S15]]
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*[[Ribosomal protein S16|Ribosomal protein S16]]
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*[[Ribosomal protein S17|Ribosomal protein S17]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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*[[Ribosomal protein S19|Ribosomal protein S19]]
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*[[Ribosomal protein S2|Ribosomal protein S2]]
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*[[Ribosomal protein S20|Ribosomal protein S20]]
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*[[Ribosomal protein S21|Ribosomal protein S21]]
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*[[Ribosomal protein S3|Ribosomal protein S3]]
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*[[Ribosomal protein S4|Ribosomal protein S4]]
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*[[Ribosomal protein S5|Ribosomal protein S5]]
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*[[Ribosomal protein S6|Ribosomal protein S6]]
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*[[Ribosomal protein S7|Ribosomal protein S7]]
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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*[[Ribosomal protein S9|Ribosomal protein S9]]
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==Reference==
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<ref group="xtra">PMID:019933110</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Armache, J.]]
[[Category: Armache, J.]]

Revision as of 00:55, 2 October 2014

E.COLI 70S RIBOSOME STALLED DURING TRANSLATION OF TNAC LEADER PEPTIDE. THIS FILE CONTAINS THE 30S, THE P-SITE TRNA AND THE TNAC LEADER PEPTIDE (PART 1 OF 2).

2wwl, resolution 5.80Å

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