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2xew

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[[Image:2xew.png|left|200px]]
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==CRYSTAL STRUCTURE OF K11-LINKED DIUBIQUITIN==
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<StructureSection load='2xew' size='340' side='right' caption='[[2xew]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xew]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XEW FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xqq|1xqq]], [[2jf5|2jf5]], [[1ubq|1ubq]], [[2gbj|2gbj]], [[2g45|2g45]], [[2gbk|2gbk]], [[1zgu|1zgu]], [[2j7q|2j7q]], [[2wdt|2wdt]], [[1f9j|1f9j]], [[2bgf|2bgf]], [[1fxt|1fxt]], [[1tbe|1tbe]], [[2ayo|2ayo]], [[1gjz|1gjz]], [[1xd3|1xd3]], [[1nbf|1nbf]], [[1g6j|1g6j]], [[1ubi|1ubi]], [[1sif|1sif]], [[1d3z|1d3z]], [[2w9n|2w9n]], [[1ogw|1ogw]], [[2gbn|2gbn]], [[2gbm|2gbm]], [[1yx5|1yx5]], [[1c3t|1c3t]], [[1q5w|1q5w]], [[1s1q|1s1q]], [[1yx6|1yx6]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xew OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xew RCSB], [http://www.ebi.ac.uk/pdbsum/2xew PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/2xew_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ubiquitin is a versatile cellular signaling molecule that can form polymers of eight different linkages, and individual linkage types have been associated with distinct cellular functions. Though little is currently known about Lys11-linked ubiquitin chains, recent data indicate that they may be as abundant as Lys48 linkages and may be involved in vital cellular processes. Here we report the generation of Lys11-linked polyubiquitin in vitro, for which the Lys11-specific E2 enzyme UBE2S was fused to a ubiquitin binding domain. Crystallographic and NMR analyses of Lys11-linked diubiquitin reveal that Lys11-linked chains adopt compact conformations in which Ile44 is solvent exposed. Furthermore, we identify the OTU family deubiquitinase Cezanne as the first deubiquitinase with Lys11-linkage preference. Our data highlight the intrinsic specificity of the ubiquitin system that extends to Lys11-linked chains and emphasize that differentially linked polyubiquitin chains must be regarded as independent post-translational modifications.
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{{STRUCTURE_2xew| PDB=2xew | SCENE= }}
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Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne.,Bremm A, Freund SM, Komander D Nat Struct Mol Biol. 2010 Aug;17(8):939-47. Epub 2010 Jul 11. PMID:20622874<ref>PMID:20622874</ref>
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===CRYSTAL STRUCTURE OF K11-LINKED DIUBIQUITIN===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20622874}}
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==About this Structure==
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[[2xew]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEW OCA].
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==See Also==
==See Also==
*[[Ubiquitin|Ubiquitin]]
*[[Ubiquitin|Ubiquitin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020622874</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Bremm, A.]]
[[Category: Bremm, A.]]

Revision as of 01:04, 2 October 2014

CRYSTAL STRUCTURE OF K11-LINKED DIUBIQUITIN

2xew, resolution 2.20Å

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