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1ire
From Proteopedia
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| - | [[Image:1ire.gif|left|200px]] | + | [[Image:1ire.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila''' | + | {{Structure |
| + | |PDB= 1ire |SIZE=350|CAPTION= <scene name='initialview01'>1ire</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IRE is a [ | + | 1IRE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudonocardia_thermophila Pseudonocardia thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRE OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:[http:// | + | Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11700034 11700034] |
[[Category: Nitrile hydratase]] | [[Category: Nitrile hydratase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: post-translational modification]] | [[Category: post-translational modification]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:53:55 2008'' |
Revision as of 09:53, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Nitrile hydratase, with EC number 4.2.1.84 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila
Overview
The crystal structure of cobalt-containing nitrile hydratase from Pseudonocardia thermophila JCM 3095 at 1.8 A resolution revealed the structure of the noncorrin cobalt at the catalytic center. Two cysteine residues (alphaCys(111) and alphaCys(113)) coordinated to the cobalt were posttranslationally modified to cysteine-sulfinic acid and to cysteine-sulfenic acid, respectively, like in iron-containing nitrile hydratase. A tryptophan residue (betaTrp(72)), which may be involved in substrate binding, replaced the tyrosine residue of iron-containing nitrile hydratase. The difference seems to be responsible for the preference for aromatic nitriles rather than aliphatic ones of cobalt-containing nitrile hydratase.
About this Structure
1IRE is a Protein complex structure of sequences from Pseudonocardia thermophila. Full crystallographic information is available from OCA.
Reference
Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:11700034
Page seeded by OCA on Thu Mar 20 11:53:55 2008
