2ztb

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[[Image:2ztb.png|left|200px]]
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==Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells==
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<StructureSection load='2ztb' size='340' side='right' caption='[[2ztb]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ztb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_dakota Bacillus thuringiensis serovar dakota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZTB FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LU:LUTETIUM+(III)+ION'>LU</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ztb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ztb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ztb RCSB], [http://www.ebi.ac.uk/pdbsum/2ztb PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zt/2ztb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Parasporin-2 is a protein toxin that is isolated from parasporal inclusions of the Gram-positive bacterium Bacillus thuringiensis. Although B. thuringiensis is generally known as a valuable source of insecticidal toxins, parasporin-2 is not insecticidal, but has a strong cytocidal activity in liver and colon cancer cells. The 37-kDa inactive nascent protein is proteolytically cleaved to the 30-kDa active form that loses both the N-terminal and the C-terminal segments. Accumulated cytological and biochemical observations on parasporin-2 imply that the protein is a pore-forming toxin. To confirm the hypothesis, we have determined the crystal structure of its active form at a resolution of 2.38 A. The protein is unusually elongated and mainly comprises long beta-strands aligned with its long axis. It is similar to aerolysin-type beta-pore-forming toxins, which strongly reinforce the pore-forming hypothesis. The molecule can be divided into three domains. Domain 1, comprising a small beta-sheet sandwiched by short alpha-helices, is probably the target-binding module. Two other domains are both beta-sandwiches and thought to be involved in oligomerization and pore formation. Domain 2 has a putative channel-forming beta-hairpin characteristic of aerolysin-type toxins. The surface of the protein has an extensive track of exposed side chains of serine and threonine residues. The track might orient the molecule on the cell membrane when domain 1 binds to the target until oligomerization and pore formation are initiated. The beta-hairpin has such a tight structure that it seems unlikely to reform, as postulated in a recent model of pore formation developed for aerolysin-type toxins. A safety lock model is proposed as an inactivation mechanism by the N-terminal inhibitory segment.
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{{STRUCTURE_2ztb| PDB=2ztb | SCENE= }}
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Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells.,Akiba T, Abe Y, Kitada S, Kusaka Y, Ito A, Ichimatsu T, Katayama H, Akao T, Higuchi K, Mizuki E, Ohba M, Kanai R, Harata K J Mol Biol. 2008 Dec 9. PMID:19094993<ref>PMID:19094993</ref>
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===Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19094993}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2ztb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_dakota Bacillus thuringiensis serovar dakota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZTB OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019094993</ref><ref group="xtra">PMID:015583389</ref><ref group="xtra">PMID:015026424</ref><references group="xtra"/>
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[[Category: Bacillus thuringiensis serovar dakota]]
[[Category: Bacillus thuringiensis serovar dakota]]
[[Category: Akiba, T.]]
[[Category: Akiba, T.]]
[[Category: Beta-hairpin]]
[[Category: Beta-hairpin]]
[[Category: Toxin]]
[[Category: Toxin]]

Revision as of 05:40, 2 October 2014

Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells

2ztb, resolution 2.38Å

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