3cm3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3cm3" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:3cm3.png|left|200px]]
+
==High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1==
 +
<StructureSection load='3cm3' size='340' side='right' caption='[[3cm3]], [[Resolution|resolution]] 1.32&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3cm3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CM3 FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2rf6|2rf6]], [[3ceo|3ceo]]</td></tr>
 +
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">H1 ORF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10245 Vaccinia virus])</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cm3 RCSB], [http://www.ebi.ac.uk/pdbsum/3cm3 PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/3cm3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The Vaccinia virus H1 gene product, VH1, is a dual specificity phosphatase that down-regulates the cellular antiviral response by dephosphorylating STAT1. The crystal structure of VH1, determined at 1.32 A resolution, reveals a novel dimeric quaternary structure, which exposes two active sites spaced approximately 39 A away from each other. VH1 forms a stable dimer via an extensive domain swap of the N-terminal helix (residues 1-20). In vitro, VH1 can dephosphorylate activated STAT1, in a reaction that is competed by the nuclear transport adapter importin alpha5. Interestingly, VH1 is inactive with respect to STAT1 bound to DNA, suggesting that the viral phosphatase acts predominantly on the cytoplasmic pool of activated STAT1. We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response.
-
{{STRUCTURE_3cm3| PDB=3cm3 | SCENE= }}
+
Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1.,Koksal AC, Nardozzi JD, Cingolani G J Biol Chem. 2009 Apr 10;284(15):10129-37. Epub 2009 Feb 10. PMID:19211553<ref>PMID:19211553</ref>
-
===High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_19211553}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[3cm3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM3 OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:019211553</ref><references group="xtra"/>
+
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
[[Category: Cingolani, G.]]
[[Category: Cingolani, G.]]

Revision as of 20:56, 2 October 2014

High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1

3cm3, resolution 1.32Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cm3"
Views
Personal tools
Navigation
Toolbox