1iyf
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1iyf.gif|left|200px]] | + | [[Image:1iyf.gif|left|200px]] |
| - | + | ||
| - | '''Solution structure of ubiquitin-like domain of human parkin''' | + | {{Structure |
| + | |PDB= 1iyf |SIZE=350|CAPTION= <scene name='initialview01'>1iyf</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of ubiquitin-like domain of human parkin''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1IYF is a [ | + | 1IYF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYF OCA]. |
==Reference== | ==Reference== | ||
| - | Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain., Sakata E, Yamaguchi Y, Kurimoto E, Kikuchi J, Yokoyama S, Yamada S, Kawahara H, Yokosawa H, Hattori N, Mizuno Y, Tanaka K, Kato K, EMBO Rep. 2003 Mar;4(3):301-6. PMID:[http:// | + | Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain., Sakata E, Yamaguchi Y, Kurimoto E, Kikuchi J, Yokoyama S, Yamada S, Kawahara H, Yokosawa H, Hattori N, Mizuno Y, Tanaka K, Kato K, EMBO Rep. 2003 Mar;4(3):301-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12634850 12634850] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 31: | Line 40: | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: ubiquitin fold]] | [[Category: ubiquitin fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:56:33 2008'' |
Revision as of 09:56, 20 March 2008
| |||||||
| Activity: | Ubiquitin--protein ligase, with EC number 6.3.2.19 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of ubiquitin-like domain of human parkin
Contents |
Overview
Parkin, a product of the causative gene of autosomal-recessive juvenile parkinsonism (AR-JP), is a RING-type E3 ubiquitin ligase and has an amino-terminal ubiquitin-like (Ubl) domain. Although a single mutation that causes an Arg to Pro substitution at position 42 of the Ubl domain (the Arg 42 mutation) has been identified in AR-JP patients, the function of this domain is not clear. In this study, we determined the three-dimensional structure of the Ubl domain of parkin by NMR, in particular by extensive use of backbone (15)N-(1)H residual dipolar-coupling data. Inspection of chemical-shift-perturbation data showed that the parkin Ubl domain binds the Rpn10 subunit of 26S proteasomes via the region of parkin that includes position 42. Our findings suggest that the Arg 42 mutation induces a conformational change in the Rpn10-binding site of Ubl, resulting in impaired proteasomal binding of parkin, which could be the cause of AR-JP.
Disease
Known diseases associated with this structure: Adenocarcinoma of lung, somatic OMIM:[602544], Adenocarcinoma, ovarian, somatic OMIM:[602544], Leprosy, susceptibility to OMIM:[602544], Parkinson disease, juvenile, type 2 OMIM:[602544]
About this Structure
1IYF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain., Sakata E, Yamaguchi Y, Kurimoto E, Kikuchi J, Yokoyama S, Yamada S, Kawahara H, Yokosawa H, Hattori N, Mizuno Y, Tanaka K, Kato K, EMBO Rep. 2003 Mar;4(3):301-6. PMID:12634850
Page seeded by OCA on Thu Mar 20 11:56:33 2008
Categories: Homo sapiens | Single protein | Ubiquitin--protein ligase | Hattori, N. | Kato, K. | Kawahara, H. | Kikuchi, J. | Kurimoto, E. | Mizuno, Y. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sakata, E. | Tanaka, K. | Yamaguchi, Y. | Yokosawa, H. | Yokoyama, S. | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Ubiquitin fold
