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2wpv

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{{STRUCTURE_2wpv| PDB=2wpv | SCENE= }}
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==CRYSTAL STRUCTURE OF S. CEREVISIAE GET4-GET5 COMPLEX==
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===CRYSTAL STRUCTURE OF S. CEREVISIAE GET4-GET5 COMPLEX===
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<StructureSection load='2wpv' size='340' side='right' caption='[[2wpv]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20106980}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wpv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WPV FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wpv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wpv RCSB], [http://www.ebi.ac.uk/pdbsum/2wpv PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wpv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Get3, Get4 and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4/Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4/Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4 and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.
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==Function==
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Crystal structure of Get4/Get5 complex and its interactions with Sgt2, Get3 and Ydj1.,Chang YW, Chuang YC, Ho YC, Cheng MY, Sun YJ, Hsiao CD, Wang C J Biol Chem. 2010 Jan 27. PMID:20106980<ref>PMID:20106980</ref>
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[[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[2wpv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WPV OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020106980</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Chang, Y W.]]
[[Category: Chang, Y W.]]

Revision as of 04:22, 3 October 2014

CRYSTAL STRUCTURE OF S. CEREVISIAE GET4-GET5 COMPLEX

2wpv, resolution 1.99Å

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