1izr
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1izr.jpg|left|200px]] | + | [[Image:1izr.jpg|left|200px]] |
| - | + | ||
| - | '''F46A mutant of bovine pancreatic ribonuclease A''' | + | {{Structure |
| + | |PDB= 1izr |SIZE=350|CAPTION= <scene name='initialview01'>1izr</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''F46A mutant of bovine pancreatic ribonuclease A''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1IZR is a [ | + | 1IZR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZR OCA]. |
==Reference== | ==Reference== | ||
| - | Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A., Kadonosono T, Chatani E, Hayashi R, Moriyama H, Ueki T, Biochemistry. 2003 Sep 16;42(36):10651-8. PMID:[http:// | + | Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A., Kadonosono T, Chatani E, Hayashi R, Moriyama H, Ueki T, Biochemistry. 2003 Sep 16;42(36):10651-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12962489 12962489] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
| Line 19: | Line 28: | ||
[[Category: Moriyama, H.]] | [[Category: Moriyama, H.]] | ||
[[Category: Ueki, T.]] | [[Category: Ueki, T.]] | ||
| - | [[Category: bovine | + | [[Category: bovine pancrea]] |
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
| - | [[Category: rnase | + | [[Category: rnase some]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:57:07 2008'' |
Revision as of 09:57, 20 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
F46A mutant of bovine pancreatic ribonuclease A
Overview
The Phe46 residue, located in the hydrophobic core of RNase A, was replaced with other hydrophobic residues, leucine, valine, or alanine, and their X-ray crystallographic structures were determined up to 1.50-1.80 A resolution in an attempt to examine the relationship between structural changes and conformational stability or folding kinetics. The backbone structure of F46L, F46V, and F46A was indistinguishable from that of the wild-type enzyme, retaining the correct active site structure. However, one water molecule was included in the hydrophobic core of F46A, forming two hydrogen bonds with the backbone peptide chain. The side chain of Met29 in F46V and F46A adopted two different conformations in an equal occupancy. A trapped water molecule and two conformations of Met29 represent changes that minimize the cavity volume. Nevertheless, the replacement of Phe46 with the above residues resulted in a marked decrease in both thermal stability and folding reaction. Thus, Phe46 ensures the thermal stability and the rapid and correct folding of RNase A by the role it plays in forming a highly packed, hydrophobic core.
About this Structure
1IZR is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A., Kadonosono T, Chatani E, Hayashi R, Moriyama H, Ueki T, Biochemistry. 2003 Sep 16;42(36):10651-8. PMID:12962489
Page seeded by OCA on Thu Mar 20 11:57:07 2008
