2jhn

From Proteopedia

Revision as of 15:29, 30 October 2007

3-METHYLADENINE DNA-GLYCOSYLASE FROM ARCHAEOGLOBUS FULGIDUS

Overview

N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic, and mutagenic lesions induced by alkylation in single-stranded DNA. In, bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired, by AlkB-mediated oxidative demethylation, a direct DNA damage reversal, mechanism. No AlkB gene homologues have been identified in Archaea. We, report that m(1)A and m(3)C are repaired by the AfAlkA base excision, repair glycosylase of Archaeoglobus fulgidus, suggesting a different, repair mechanism for these lesions in the third domain of life. In, addition, AfAlkA was found to effect a robust excision of, 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of, AfAlkA, which, together with the characterization of several site-directed, mutants, forms a ... [(full description)]

About this Structure

2JHN is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with HG, NA, MBO, MES and GOL as [ligands]. Active as [DNA-3-methyladenine glycosylase II], with EC number [3.2.2.21]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA., Leiros I, Nabong MP, Grosvik K, Ringvoll J, Haugland GT, Uldal L, Reite K, Olsbu IK, Knaevelsrud I, Moe E, Andersen OA, Birkeland NK, Ruoff P, Klungland A, Bjelland S, EMBO J. 2007 Apr 18;26(8):2206-17. Epub 2007 Mar 29. PMID:17396151

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