1oxe

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[[Image:1oxe.png|left|200px]]
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==Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins==
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<StructureSection load='1oxe' size='340' side='right' caption='[[1oxe]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1oxe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1009 Cfp marker plasmid pwm1009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OXE FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRF:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CRF</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1emb|1emb]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oxe RCSB], [http://www.ebi.ac.uk/pdbsum/1oxe PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.
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{{STRUCTURE_1oxe| PDB=1oxe | SCENE= }}
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Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins.,Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N J Mol Biol. 2003 May 16;328(5):1071-81. PMID:12729742<ref>PMID:12729742</ref>
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===Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_12729742}}
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==About this Structure==
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[[1oxe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1009 Cfp marker plasmid pwm1009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXE OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
 
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
 
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Sandbox104|Sandbox104]]
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== References ==
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:012729742</ref><references group="xtra"/>
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[[Category: Cfp marker plasmid pwm1009]]
[[Category: Cfp marker plasmid pwm1009]]
[[Category: Azim, M K.]]
[[Category: Azim, M K.]]

Revision as of 05:19, 3 October 2014

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

1oxe, resolution 1.15Å

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