2cvq

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{{STRUCTURE_2cvq| PDB=2cvq | SCENE= }}
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==Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH==
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===Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH===
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<StructureSection load='2cvq' size='340' side='right' caption='[[2cvq]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
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{{ABSTRACT_PUBMED_16009341}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cvq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermus_thermophilus"_oshima_and_imahori_1974 "thermus thermophilus" oshima and imahori 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CVQ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bmd|1bmd]], [[1y7t|1y7t]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cvq RCSB], [http://www.ebi.ac.uk/pdbsum/2cvq PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/2cvq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.
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==Function==
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Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H).,Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. PMID:16009341<ref>PMID:16009341</ref>
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[[http://www.uniprot.org/uniprot/MDH_THETH MDH_THETH]] Catalyzes the reversible oxidation of malate to oxaloacetate.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[2cvq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermus_thermophilus"_oshima_and_imahori_1974 "thermus thermophilus" oshima and imahori 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA].
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</div>
==See Also==
==See Also==
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*[[Johnson sandbox 1|Johnson sandbox 1]]
 
*[[Malate dehydrogenase|Malate dehydrogenase]]
*[[Malate dehydrogenase|Malate dehydrogenase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016009341</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Thermus thermophilus oshima and imahori 1974]]
[[Category: Thermus thermophilus oshima and imahori 1974]]
[[Category: Malate dehydrogenase]]
[[Category: Malate dehydrogenase]]

Revision as of 05:34, 3 October 2014

Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH

2cvq, resolution 2.08Å

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