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1j2x

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[[Image:1j2x.jpg|left|200px]]<br /><applet load="1j2x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1j2x.jpg|left|200px]]
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caption="1j2x, resolution 2.00&Aring;" />
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'''Crystal structure of RAP74 C-terminal domain complexed with FCP1 C-terminal peptide'''<br />
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{{Structure
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|PDB= 1j2x |SIZE=350|CAPTION= <scene name='initialview01'>1j2x</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE= RAP74 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal structure of RAP74 C-terminal domain complexed with FCP1 C-terminal peptide'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1J2X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2X OCA].
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1J2X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2X OCA].
==Reference==
==Reference==
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Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF., Kamada K, Roeder RG, Burley SK, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2296-9. Epub 2003 Feb 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12591941 12591941]
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Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF., Kamada K, Roeder RG, Burley SK, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2296-9. Epub 2003 Feb 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12591941 12591941]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: winged-helix domain]]
[[Category: winged-helix domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:18:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:58:10 2008''

Revision as of 09:58, 20 March 2008

Image:1j2x.jpg


PDB ID 1j2x

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: and
Gene: RAP74 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of RAP74 C-terminal domain complexed with FCP1 C-terminal peptide


Contents

Overview

After mRNA transcription termination in eukaryotes, the hyperphosphorylated form of RNA polymerase II (pol II0) must be recycled by TFIIF-associating C-terminal domain phosphatase (FCP1), the phosphatase responsible for dephosphorylating the C-terminal domain of the largest polymerase subunit. Transcription factor (TF)-IIF stimulates the activity of FCP1, and the RNA polymerase II-associating protein 74 subunit of TFIIF forms a complex with FCP1 in both human and yeast. Here, we report a cocrystal structure of the winged-helix domain of human RNA polymerase II-associating protein 74 bound to the alpha-helical C terminus of human FCP1 (residues 944-961). These results illustrate the molecular mechanism by which TFIIF efficiently recruits FCP1 to the pol II transcription machinery for recycling of the polymerase.

Disease

Known disease associated with this structure: Congenital cataracts, facial dysmorphism, and neuropathy OMIM:[604927]

About this Structure

1J2X is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF., Kamada K, Roeder RG, Burley SK, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2296-9. Epub 2003 Feb 18. PMID:12591941

Page seeded by OCA on Thu Mar 20 11:58:10 2008

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