2cnb

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{{STRUCTURE_2cnb| PDB=2cnb | SCENE= }}
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==TRYPANOSOMA BRUCEI UDP-GALACTOSE-4-EPIMERASE IN TERNARY COMPLEX WITH NAD AND THE SUBSTRATE ANALOGUE UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE==
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===TRYPANOSOMA BRUCEI UDP-GALACTOSE-4-EPIMERASE IN TERNARY COMPLEX WITH NAD AND THE SUBSTRATE ANALOGUE UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE===
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<StructureSection load='2cnb' size='340' side='right' caption='[[2cnb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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{{ABSTRACT_PUBMED_16946458}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cnb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CNB FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UFG:URIDINE-5-DIPHOSPHATE-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE'>UFG</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1gy8|1gy8]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_4-epimerase UDP-glucose 4-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.2 5.1.3.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cnb RCSB], [http://www.ebi.ac.uk/pdbsum/2cnb PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the NAD-dependent oxidoreductase UDP-galactose-4'-epimerase from Trypanosoma brucei in complex with cofactor and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose has been determined using diffraction data to 2.7 A resolution. Despite the high level of sequence and structure conservation between the trypanosomatid enzyme and those from humans, yeast and bacteria, the binding of the 4-fluoro-alpha-D-galactose moiety is distinct from previously reported structures. Of particular note is the observation that when bound to the T. brucei enzyme, the galactose moiety of this fluoro-derivative is rotated approximately 180 degrees with respect to the orientation of the hexose component of UDP-glucose when in complex with the human enzyme. The architecture of the catalytic centre is designed to effectively bind different orientations of the hexose, a finding that is consistent with a mechanism that requires the sugar to maintain a degree of flexibility within the active site.
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==About this Structure==
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Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose.,Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):829-34. Epub 2006 Aug 11. PMID:16946458<ref>PMID:16946458</ref>
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[[2cnb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNB OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]]
*[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016946458</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Trypanosoma brucei]]
[[Category: Trypanosoma brucei]]
[[Category: UDP-glucose 4-epimerase]]
[[Category: UDP-glucose 4-epimerase]]

Revision as of 09:22, 3 October 2014

TRYPANOSOMA BRUCEI UDP-GALACTOSE-4-EPIMERASE IN TERNARY COMPLEX WITH NAD AND THE SUBSTRATE ANALOGUE UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE

2cnb, resolution 2.70Å

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